2014 Fiscal Year Final Research Report
Transcriptional analysis of Chlamydia psittaci in host cells.
Project/Area Number |
24658255
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Applied veterinary science
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Research Institution | Gifu University |
Principal Investigator |
OHYA Kenji 岐阜大学, 応用生物科学部, 准教授 (50402219)
|
Co-Investigator(Kenkyū-buntansha) |
FUKUSHI Hideto 岐阜大学, 応用生物科学部, 教授 (10156763)
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | 獣医学 / 病原細菌 / 偏性細胞内寄生細菌 / クラミジア / 細胞内増殖 / トランスクリプトーム |
Outline of Final Research Achievements |
Chlamydia, an obligate intracellular pathogen, possesses the peculiar developmental cycle involving biological conversion in host cells. In this study, aiming at revealing the regulatory mechanism of Chlamydial developmental cycle, transcriptional analysis (RNAseq) of Chlamydia in host cells was performed. There were few reports about RNA-seq analysis of obligate intracellular pathogens at that time. Prior to the RNA-seq analysis, we attempted enrichment of Chlamydial mRNA and depletion of host/Chlamydial rRNA in samples. Although novel transcribed regions including ncRNA were not identified, we obtained important information regarding about dealing with RNA of obligate intracellular pathogens toward RNA-seq. The RNA-seq of Chlamydia is ongoing in the continuous project.
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Free Research Field |
獣医微生物学
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