The research on flavivirus organ-tropism

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658261
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Kyoto Sangyo University
• Principal Investigator: MAEDA Akihiko 京都産業大学, 総合生命科学部, 教授 (70333359)
• Co-Investigator(Kenkyū-buntansha): SASAKI Nobuya 北里大学, 獣医学部, 教授 (20302614)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 感染症 / フラビウイルス / スライス培養 / 臓器特異性

Research Abstract

In order to solidify organ-tropisms of pathogenic microbes, we conducted the experiments as described below, using a neurotropic flavivirus as a model of infectious agent. First, flavivirus reporter virus particles (RVPs) encoding the red-fluorescent protein gene within its genome RNA, were prepared to be visible virus infections. Next, mouse brain slice-culture system was established to analyze organ-tropism of the virus easily. Finally, flavivirus RVPs-infection system on mouse brain slice-cultures was established. We might apply the system to do research on organ-tropism of pathogenic microbes.

Research Products

• [Journal Article] 京都市市街地における蚊の調査 (2014)
  • Author(s): 伊藤亜希,岡本奈津実,米島万有子,染谷梓,前田秋彦
  • Journal Title: 京都産業大学総合学術研究所所報 Volume: 9巻(In press)
• [Journal Article] Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport (2013)
  • Author(s): Makino, Y., Suzuki, T., Hasebe, R., Kimura, T., and Maeda, A., Takahashi, H., Sawa, H.
  • Journal Title: J. Virol. Methods Volume: 195 Pages: 250-257
  • DOI: 10.1016/j.jviromet.2013.10.002
  • Peer Reviewed
• [Journal Article] Review of diagnostic plaque reduction neutralization tests for flavivirus infection (2013)
  • Author(s): Maeda, A., and Maeda, J
  • Journal Title: Vet. J Volume: 195 Pages: 33-40
  • DOI: 10.1016/j.tvjl.2012.08.019
  • Peer Reviewed
• [Journal Article] 京都市山科区で駆除されたイノシシに寄生していたマダニ類の解析 (2013)
  • Author(s): 染谷梓,池永充宏,大西修,Igor Velado Fernandez,西野佳以,前田秋彦
  • Journal Title: 京都産業大学総合学術研究所所報 Volume: 8巻 Pages: 57-62
• [Journal Article] A. The chicken 2-5 oligoadenylate synthetase A inhibits the replication of West Nile Virus (2012)
  • Author(s): Tag-El-din-Hassan, Hassan T., Sasaki, N., Moritoh, K., Torigoe, D., Maeda, A., and Takashi
  • Journal Title: Jap. J. Vet. Res Volume: 60 Pages: 95-103
  • Peer Reviewed
• [Presentation] Effects of land use pattern on spatial distribution of host-seeking mosquitoes within urban areas in Kyoto, Japan (2013)
  • Author(s): Yonejima, M., Nakaya, T., Nihei, N., Tsuda, Y., Koboyashi, M., Watanabe, M. and Maeda, A
  • Organizer: International Geographic Union, Kyoto Regional Conference
  • Place of Presentation: Kyoto
  • Year and Date: 20130804-09
• [Presentation] 京都市における蚊媒介性フラビウイルス媒介蚊の調査 (2013)
  • Author(s): 伊藤亜希,米島万有子, Igor Velado Fernandez,福田美樹,染谷梓,前田秋彦
  • Organizer: 第48回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 熱海市
  • Year and Date: 20130524-25
• [Presentation] 京都市におけるアカイエカとチカイエカの捕集数および構成比の空間差異 (2013)
  • Author(s): 米島万有子,前田秋彦,福田美樹,伊藤亜希,Igor Velado Fernandez,津田良夫,渡辺護,中谷友樹
  • Organizer: 第65回日本衛生動物学会
  • Place of Presentation: 江別市
  • Year and Date: 20130405-07
• [Presentation] 京都市における紅斑熱群リケッチアの検出 (2012)
  • Author(s): 染谷梓,池永充宏,大西修,近野真由美,杉江真理子,Igor Velado Fernandez,西野佳以,前田秋彦
  • Organizer: 第154回日本獣医学会総会
  • Place of Presentation: 盛岡市
  • Year and Date: 20120914-16
• [Book] 第4章動物感染症診断のための微生物検査,1.産業動物,4)神経系感染症,「動物微生物検査学」 (2014)
  • Author(s): 前田秋彦((編)副所秋雄,前田秋彦ら)
  • Total Pages: 155-157
  • Publisher: 近代出版