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2013 Fiscal Year Final Research Report

Generation of hematopoietic cells from canine induced pluripotent stem cells for cell transplantation

Research Project

  • PDF
Project/Area Number 24658270
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Clinical veterinary science
Research InstitutionOsaka Prefecture University

Principal Investigator

INABA TOSHIO  大阪府立大学, 生命環境科学研究科(系), 教授 (00137241)

Co-Investigator(Kenkyū-buntansha) SHUGIURA Kikuya  大阪府立大学, 生命環境科学研究科, 准教 (30171143)
HATOYA Shingo  大阪府立大学, 生命環境科学研究科, 助教 (40453138)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywords再生医療 / 統合動物科学 / iPS細胞 / イヌ / サイトカイン / バイオテクノロジー / トランスレーショナルリサーチ
Research Abstract

The objective of this study was in the development of the effective method for generation of hematopoietic cells from canine induced pluripotent stem (iPS) cells for cell transplantation, and the following results were obtained. We successfully cloned three canine hematopoietic cytokine genes and produced their proteins. We reprogrammed adult dog skin fibroblasts into iPS-like cells, using 4 transcription factors. While the cell colonies showed negative alkaline phosphatase activity, they were maintained until 24 passages. Erythroblasts could be induced from feline iPS cells, using several hematopoietic cytokines. This method will apply to canine iPS cells.

  • Research Products

    (13 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] Evaluation of serum phosphorylated neurofilament dubunit NF-H as a prognostic biomarker in dogs with thoracolumbar intervertebral disc herniation2014

    • Author(s)
      Nishida, H., Nakayama, M., Tanaka, H., Kamishina, H., Izawa, T., Hatoya, S., Sugiura, K., Suzuki, Y., Ide, C., Inaba, T.
    • Journal Title

      Veterinary Surgery

      Volume: 43巻 Pages: 289-293

    • DOI

      10.1111/j.1532-950X.2014.12144.x

    • Peer Reviewed
  • [Journal Article] Tamada, H. Clinical study report on milk production in the offspring of a somatic cell cloned Holstein Cow2013

    • Author(s)
      Takahashi, M., Tsuchiya, H., Hamano, S., Inaba, T., Kawate, N.
    • Journal Title

      Journal of Reproduction and Developmen

      Volume: 59巻 Pages: 595-597

    • DOI

      10.1262/jrd.2013-025

    • Peer Reviewed
  • [Journal Article] Improvement of superovulatory response and pregnancy rate after transfer of embryos recovered from Japanese black cows fed rumen bypass polyunsaturated fatty acids2013

    • Author(s)
      Takahashi, M., Sawada, K., Kawate, N., Inaba, T., Tamada, H.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 75巻 Pages: 1485-1490

    • DOI

      10.1262/jrd.2013-025

    • Peer Reviewed
  • [Journal Article] Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy2013

    • Author(s)
      Wijewardana, V., Sugiura, K., Yahata, M., Akazawa, T., Wijesekera, D.P., Imamoto, S., Hatoya, S., Inoue, N., Inaba, T.
    • Journal Title

      Veterinary Immunology and Immunopathology

      Volume: 156巻 Pages: 121-127

    • DOI

      10.1016/j.vetimm.2013.09.016

    • Peer Reviewed
  • [Journal Article] Generation of functional platelets from canine induced pluripotent stem cells2013

    • Author(s)
      Nishimura, T., Hatoya, S., Kanegi, R., Sugiura, K., Wijewardana, V., Kuwamura, M., Tanaka, M., Yamate, J., Izawa, T., Takahashi, M., Kawate, N., Tamada, H., Imai, H., Inaba, T
    • Journal Title

      Stem Cells and Development

      Volume: 22巻 Pages: 2026-2035

    • DOI

      10.1089/scd.2012.0701

    • Peer Reviewed
  • [Presentation] Improvement of dendritic cell-based immunotherapy for enhancing systemic immune responses against cancer2013

    • Author(s)
      Wijewardana, V., Sugiura, K., Akazawa, T., Wijesekera, D.-P.H., Imamoto, S., Inoue, N., Inaba, T.
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      千葉
    • Year and Date
      2013-12-12
  • [Presentation] イヌiPS細胞から神経系細胞への分化誘導培養系に及ぼす骨髄間質細胞の影響2013

    • Author(s)
      因野由衣子、西村俊哉、西田英高、鳩谷晋吾、杉浦喜久弥、川手憲俊、高橋正弘、玉田尋通、稲葉俊夫
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Year and Date
      2013-09-20
  • [Presentation] イエネコ体細胞を用いた人工多能性幹細胞の誘導2013

    • Author(s)
      古田侑吾、川口高正、南直治郎、山田雅保、稲葉俊夫、今井裕
    • Organizer
      第63回関西畜産学会
    • Place of Presentation
      滋賀
    • Year and Date
      2013-09-05
  • [Presentation] Generation of functional platelets from canine induced pluripotent stem cells2013

    • Author(s)
      Nishimura, T., Hatoya, S., Kanegi, R., Sugiura, K., Wijewardana, V., Kuwamura, M., Tanaka, M., Takahashi, M., Kawate, N., Tamada, H., Imai, H., Inaba, T.
    • Organizer
      The International Society for Stem Cell Research (ISSCR) 11th Annual Meeting
    • Place of Presentation
      米国ボストン
    • Year and Date
      2013-06-15
  • [Presentation] Generation of induced pluripotent stem cells and production of leukemia inhibitory factor (LIF) in domestic cats2013

    • Author(s)
      Kanegi, R., Hatoya, S., Takenaka, S., Nishimura, T., Wijewardana, V., Sugiura, K., Takahashi, M., Kawate, N., Tamada, H., Inaba, T.
    • Organizer
      The International Society for Stem Cell Research (ISSCR) 11th Annual Meeting
    • Place of Presentation
      米国ボストン
    • Year and Date
      2013-06-14
  • [Presentation] ネコiPS細胞株樹立と多能性維持タンパク質ネコLIFの作製2012

    • Author(s)
      金城綾二、鳩谷晋吾、杉浦喜久弥、竹中重雄、玉田尋通、川手憲俊、高橋正弘、稲葉俊夫
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手
    • Year and Date
      2012-09-14
  • [Presentation] イヌiPS細胞の作製と血小板への分化誘導2012

    • Author(s)
      西村俊哉、鳩谷晋吾、杉浦喜久弥、今井裕、玉田尋通、川手憲俊、高橋正弘、稲葉俊夫
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手
    • Year and Date
      2012-09-14
  • [Remarks]

    • URL

      http://kyoindb.acs.osakafu-u.ac.jp/profile/out.cudjDYHfHMO.PEVGDxV-iw==.html

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Published: 2015-06-25  

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