2014 Fiscal Year Final Research Report
Innovation of plant biomass production by identifying master genes for amyloplast differentiation and dedifferentiation
| Project/Area Number |
24658271
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Boundary agriculture
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAZAWA Yutaka 山形大学, 理学部, 准教授 (00342858)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | アミロプラスト / 分化 / 脱分化 |
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| Outline of Final Research Achievements |
To investigate the molecular mechanism of amyloplast differentiation and dedifferentiation, we first intended to establish experimental system that can reproducibly induce amyloplast differentiation and dedifferentiation. Treating Arabidopsis seedlings with 0.4 M mannitol solution, we found that amyloplasts at their root caps degraded in 4 hrs. Furthermore, we found that transferring these amyloplast-less seedlings resulted in the recovery of amyloplasts. Together with other results, we concluded that the treatments described above are suitable for analyzing the physiological and molecular biological nature of amylolplast differentiation and dedifferentiation. Next, we screened genes that are closely related with amyloplast differentiation and dedifferentiation. We referred to the public transcriptome database and successfully identified 19 genes as candidates. Of these genes, two genes were picked up as molecular markers of amyloplast differentiation and dedifferentiation.
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| Free Research Field |
植物生理学
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