Establishment of an efficient TALE-mediated genome editing in plants

2014 Fiscal Year Final Research Report

• Project/Area Number: 24658283
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Ritsumeikan University (2013-2014); The University of Tokyo (2012)
• Principal Investigator: TAKEDA Atsushi 立命館大学, 生命科学部, 准教授 (60560779)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ゲノム編集

Outline of Final Research Achievements

In this study, we established a Transcription Activator-Like Effector Nuclease (TALEN) system, which efficiently functions in plants. We first generated a TALE backbone and repeats in the TALE DNA binding domain according to the codon usage of a model plant, Arabidopsis thaliana. Our TALE system has several advantages compared to other plant TALEN systems previously reported. In addition, we developed a transient Luciferase (Luc) assay system, in which we are able to evaluate the efficiency of the binding ability of the assembled TALEs with the target DNA sequences in plants. By using this Luc assay system, we confirmed the abilities of the assembled TALENs before we start to generate transgenic plants. We successfully introduced deletion mutations by using our TALEN system in plants. The systems we developed in this study help contribute to the advances in plant research in future.

Free Research Field

植物遺伝子工学