The experimental production of the protein transport system to the cytoplasm.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658284
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: NISHIKAWA Atsushi 東京農工大学, (連合)農学研究科(研究院), 教授 (30218127)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ボツリヌス毒素 / ドラッグ・デリバリー・システム

Research Abstract

Botulinum progenitor toxin consists of one neurotoxin (NTX) and multiple non-toxic components including several haemagglutinin (HA) proteins. Orally ingested NTX passes through the epithelial layer into the blood circulation system with the aide of HA components, and specifically binds to the peripheral nerve cells, and invades into cytoplasm, then causes food-borne botulism. In this study, we challenged the development of novel drug delivery system (DDS) utilizing the botulinum tactical infection mechanism, and a several results which provide a platform for novel DDS were obtained.

Research Products

• [Journal Article] Sugar-induced conformational change found in the HA-33/HA-17 trimer of the botulinum toxin complex (2013)
  • Author(s): Yoshimasa Sagane, Shintaro Hayash, Takashi Matsumoto, Shin-ichro Miyashita, Ken Inui, Keita Miyata, Shunsuke Yajima, Tomonori Suzuki, Kimiko Hasegawa, Akihito Yamano, Atushi Nishikawa, Tohru Ohyama, Toshihiro Watanabe, Koichi Niwa
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 438 Pages: 483-487
  • DOI: 10.1016/j.bbrc.2013.07.112
  • Peer Reviewed
• [Journal Article] Purification, crystallization and preliminary X-ray analysis of an HA17-HA70 (HA2-HA3) complex from Clostridium botulinum type C progenitor Toxin
  • Author(s): Chikako Iwasa,Takashi Tonozuka, Masaya Shinoda,Yoshimasa Sagane, Koichi Niwa, Toshihiro Watanabe, Hiromi Yoshida, Shigehiro Kamitori, Toshifumi Takao, Keiji Oguma, Atsushi Nishikawa
  • Journal Title: Acta Crystallographia Volume: F70 Pages: 64-67
  • DOI: 10.1107/S2053230X13032378
  • Peer Reviewed
• [Presentation] C型ボツリヌス毒素複合体構成成分HA2-3複合体の構造と機能の解析 (2013)
  • Author(s): 岩佐知可子、殿塚隆史、篠田昌也、中村智美、中北愼一、神鳥成弘、小熊惠二、西河淳
  • Organizer: 日本生化学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20130911-13
• [Book] Traffic of botulinum toxin complex, Glycoscience : Biology and Medicine (2014)
  • Author(s): Takashi Tonozuka, Keiji Oguma, Atsushi Nishikawa(Naoyuki Taniguchi, Tamao Endo, Gerald Hart, Peter Seeberger, Chi-Huey Wong (Eds.))
  • Publisher: Springer(in press)