2013 Fiscal Year Final Research Report
Control of gene amplification-rate in animal cells, and its application
| Project/Area Number |
24658290
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 遺伝子増幅 / 組換え蛋白質生産 / 染色体外遺伝因子 / 細胞がん化 / 細胞工学 / 哺乳動物複製開始 |
|---|
| Research Abstract |
We examined how the IR/MAR sequence, which promote replication initiation, accelerate the gene amplification that was stimulated by the Mtx treatment. Consequently, we uncovered that the IR/MAR sequence enable the initial extrachromosomal maintenance and multiplication of the introduced sequence, which allowed its integration to the multiple chromosomal loci that might be proximity to the chromosomal fragile sites. Thus, the target sequence might efficiently be amplified at the chromosomal context by the BFB cycle, after the Mtx treatments. Our study uncovered the gene amplification mechanism during cancer development. Furthermore, it provided the mechanistic base for the efficient production of protein pharmaceuticals.
|
Research Products
(8 results)
