2013 Fiscal Year Final Research Report
Development of novel nano discs for structural analysis of membrane proteins
| Project/Area Number |
24659016
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YANO Yoshiaki 京都大学, 大学院薬学研究科, 助教 (60402799)
HOSHINO Masaru 京都大学, 大学院薬学研究科, 准教授 (70304053)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 膜蛋白質 / 可溶化剤 |
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| Research Abstract |
New agents for solubilization of membrane proteins were designed to cover hydrophobic surface of proteins with bilayer-like orientations. A phosphatidylcholine-based solubilizing agent was synthesized by substituting one acyl chain with a detergent. The agent was found to stably solubilize bacteriorhodopsins with lower denaturing effect compared with conventional detergents. Only ~30 molecules were required for solubilization of one bacteriophodopsin. These promising results indicate that the agent has superior properties for structural studies of membrane proteins.
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