2013 Fiscal Year Final Research Report
Enrichment of low abundant proteome in plasma
| Project/Area Number |
24659017
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
WAKABAYASHI Masaki 京都大学, 大学院薬学研究科, 助教 (70552024)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | プロテオーム / 血漿プロテオーム |
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| Research Abstract |
We aimed to develop a novel method to enrich low abundant proteins, in which target proteins are labeled with phosphate by in vitro phosphorylation reaction using selected spiked kinases, followed by phosphopeptide enrichment, for applying to plasma/serum proteome analysis. As a result, by combining the in vitro phosphorylation by optimal kinase with sample treatment by antibody-based serum depletion column, it becomes possible to identify several cytokines with very low expression level in plasma, which have never been reported to identify from plasma by conventional proteomics approaches due to their low abundance. Furthermore, we successfully quantified low abundant phosphorylated proteins in cultured cells by this method, which were not possible by the conventional methods.
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[Journal Article] Discovery of colorectal cancer biomarker candidates by membrane proteomic analysisand subsequent verification using selected reaction monitoring and tissue microarrayanalysis2014
Author(s)
Kume, H.; Muraoka, S.; Kuga, T.; Adachi, J.; Narumi, R.; Watanabe, S.; Kuwano, M.; Kodera, Y.; Matsushita, K.; Fukuoka, J.; Masuda, T.; Ishihama, Y..; Matsubara, H.; Nomura, F.; Tomonaga, T.
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Journal Title
Mol Cell Proteomics
Volume: M113
Pages: 037093
DOI
Peer Reviewed
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[Journal Article] Metabolomic profiling of lung and prostate tumor tissues by capillary electrophoresis time-of-flight mass spectrometry2013
Author(s)
Kami, K.; Fujimori, T.; Sato, H.; Sato, M.; Yamamoto, H.; Ohashi, Y.; Sugiyama, N.; Ishihama, Y..; Onozuka, H.; Ochiai, A.; Esumi, H.; Soga, T.; Tomita, M.
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Journal Title
Metabolomics
Volume: 9 (2)
Pages: 444-453
DOI
Peer Reviewed
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[Journal Article] Phosphoproteomic analysis of Rhodopseudomonas palustris reveals the role of pyruvate phosphate dikinase phosphorylation in lipid production2012
Author(s)
Hu, C. W.; Lin, M. H.; Huang, H. C.; Ku, W. C.; Yi, T. H.; Tsai, C. F.; Chen, Y. J.; Sugiyama, N.; Ishihama, Y..; Juan, H. F.; Wu, S. H.
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Journal Title
J Proteome Res
Volume: 11 (11)
Pages: 5362-75
DOI
Peer Reviewed
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[Journal Article] LATS1/WARTS phosphorylates MYPT1 to counteract PLK1 and regulate mammalian mitotic progression2012
Author(s)
Chiyoda, T.; Sugiyama, N.; Shimizu, T.; Naoe, H.; Kobayashi, Y.; Ishizawa, J.; Arima, Y.; Tsuda, H.; Ito, M.; Kaibuchi, K.; Aoki, D.; Ishihama, Y..; Saya, H.; Kuninaka,S.
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Journal Title
J Cell Biol
Volume: 197 (5)
Pages: 625-41
Peer Reviewed
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[Journal Article] Short-chain fatty acid receptor GPR41-mediated activation of sympathetic neurons involves synapsin 2b phosphorylation2012
Author(s)
Inoue, D.; Kimura, I.; Wakabayashi, M..; Tsumoto, H.; Ozawa, K.; Hara, T.; Takei, Y.; Hirasawa, A.; Ishihama, Y..; Tsujimoto, G.
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Journal Title
FEBS Lett
Volume: 586 (10)
Pages: 1547-54
DOI
Peer Reviewed
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