2013 Fiscal Year Final Research Report
Development and evaluation of fluorescence probe response to environment and lipid derived radicals
| Project/Area Number |
24659020
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
YAMADA Kenichi 九州大学, 薬学研究科(研究院), 准教授 (60346806)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 生物物理化学 |
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| Research Abstract |
Lipid derived radicals generated by reactive oxygen species produce the lipid peroxidation resulting in cell damage. Moreover the end products of lipid peroxidation were reported to have mutagenicity. Therefore, to develop the lipid radical detection method is important. However, the detection technique with high sensitive is not yet established. We have been developed the synthetic method to substitute the methyl group of piperidine nitroxides at 2,6-position to give the selectivity. In this study, we aimed to develop and evaluate the fluorescence probe to detect lipid radicals under hydrophobic condition based on interaction between fluorophore and nitroxide.
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