2013 Fiscal Year Final Research Report
Biological roles of fatty acid composition of phospholipids.
| Project/Area Number |
24659025
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
AOKI Junken 東北大学, 薬学研究科(研究院), 教授 (20250219)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | PLA1 / fatty acid / massspectrometry / remodeling |
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| Research Abstract |
In this study, we examined the mechanism of fatty acid remodeling and its biological roles. We focused two phospholipases called iPLA1a and iPLA1g. We knockdowned the two iPLA1s by RNAi technique in HeLa cells and examined the fatty acid composition of the cells. We found that fatty acid remodening of phospholipid at the sn-1 position was dramatically delayed in iPLA1a-knockdowned HeLa cells. In addition, abnormal mitochondria were frequently observed in the iPLA1a-knockdowned cells. These results indicate that fatty acid remodeling at the sn-1 position of phospholipids by iPLA1a has a critical role in the function of mitochondria.
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