2013 Fiscal Year Final Research Report
A search for novel histidine-based protein phosphatases and structural analysis of their substrate specificities
| Project/Area Number |
24659027
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
TAKEDA Kohsuke 長崎大学, 医歯(薬)学総合研究科, 教授 (10313230)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | シグナル伝達 / ストレス応答 / プロテインホスファターゼ |
|---|
| Research Abstract |
We have recently identified PGAM5 as a novel type of protein phosphatase that relies on a histidine residue as a catalytic center; however, its mechanism of enzymatic action has not been revealed. In the current study, we found by structural analyses that the catalytic center of PGAM5 consists of two histidines and two arginines. We also found that PGAM5 forms a dimer and that the dimerization is required for the catalytic activity of PGAM5. These results provide important clues to the mechanism of action of the histidine-based protein phosphatases.
|
-
[Journal Article] The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts2014
Author(s)
Matsui, H., Fukuno, N., Kanda, Y., Kantoh, Y., Chida, T., Nagaura, Y., Suzuki, O., Nishitoh, H., Takeda, K., Ichijo, H., Sawada, Y., Sasaki, K., Kobayashi, T. and Tamura, S.
-
Journal Title
J. Biol. Chem.
Volume: 289
Pages: 6138-6450
Peer Reviewed
-
[Journal Article] SOD1 as a molecular switch for initiating the homeostatic ER stress response under zinc deficiency2013
Author(s)
Homma, K., Fujisawa, T., Tsuburaya, N., Yamaguchi, N., Kadowaki, H., Takeda, K., Nishitoh, H., Matsuzawa, A., Naguro, I. and Ichijo, H.
-
Journal Title
Mol. Cell
Volume: 52
Pages: 75-86
Peer Reviewed
-
[Journal Article] Involvement of ASK1-p38 pathway in the pathogenesis of diabetes triggered by pancreaticβcell exhaustion2013
Author(s)
Yamaguchi, K., Takeda, K., Kadowaki, H., Ueda, I., Namba, Y., Ouchi, Y., Nishitoh, H. and Ichijo, H.
-
Journal Title
Biochim. Biophys. Acta
Volume: 1830
Pages: 3656-3663
Peer Reviewed
-
-
-
-
-
[Journal Article] The kelch repeat protein KLHDC10 regulates oxidative stress-induced ASK1 activation by suppressing PP52012
Author(s)
Sekine, Y., Hatanaka, R., Watanabe, T., Sono, N., Iemura, S., Natsume, T., Kuranaga, E., Miura, M., Takeda, K. and Ichijo, H.
-
Journal Title
Mol. Cell
Volume: 48
Pages: 692-704
Peer Reviewed
-
[Journal Article] Apoptosis signal-regulating kinase -1 inhibitor as a potent therapeutic drug for the treatment of gastric cancer2012
Author(s)
Hayakawa, Y., Hirata, Y., Sakitani, K., Nakagawa, H., Nakata, W., Kinoshita, H., Takahashi, R., Takeda, K., Ichijo, H., Maeda, S. and Koike, K.
-
Journal Title
Cancer Sci
Volume: 103
Pages: 2181-2185
Peer Reviewed
-
[Journal Article] Rhomboid protease PARL mediates the mitochondrial membrane potential loss-induced cleavage of PGAM52012
Author(s)
Sekine, S., Kanamaru, Y., Koike, M., Nishihara, A., Okada, M., Kinoshita, H., Kamiyama, M., Maruyama, J., Uchiyama, Y., Ishihara, N., Takeda, K. and Ichijo, H.
-
Journal Title
J. Biol. Chem.
Volume: 287
Pages: 34635-34645
Peer Reviewed
-
[Journal Article] A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral sclerosis-linked SOD1 mutants2012
Author(s)
Fujisawa, T., Homma, K., Yamaguchi, N., Kadowaki, H., Tsuburaya, N., Naguro, I., Matsuzawa, A., Takeda, K., Takahashi, Y., Goto, J., Tsuji, S., Nishitoh, H. and Ichijo, H.
-
Journal Title
Ann. Neurol.
Volume: 72
Pages: 739-749
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] Roles for the stress-responsive kinases ASK1 and ASK2 in tumorigenesis. Chembiomolecular Science : At the Frontier of Chemistry and Biology.2012
Author(s)
Kamiyama, M., Sato, T., Takeda, K. and Ichijo, H.(eds Shibasaki, M., Iino, M. and Osada, H)
Total Pages
145-154
Publisher
Springer Japan
-
-
