2013 Fiscal Year Final Research Report
Establishment of screening system for antifungal drugs using silkworm fungus infection model
| Project/Area Number |
24659041
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Drug development chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HAMAMOTO Hiroshi 東京大学, 大学院薬学系研究科, 助教 (90361609)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 真菌 / 動物モデル / カイコ / 体内動態 |
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| Research Abstract |
When Aspergillus fumigatus and Candida krusei was injected into hemolymph, all silkworms were killed. Furthermore, antifungal agents showed quantitative therapeutic effect and gave similar ED50 values against silkworm A. fumigatus infection model. We next analyzed drug concentration after injection of antifungals into hemolymph of silkworm. These antifungals in silkworm hemolymph could be explained by two-compartment model. In addition, antifungal activities of externally used antifungals were lost by addition of hemolymph, and these antifungals showed low therapeutic activity in silkworm infection models. Meanwhile, antifungal activity of fluconazole, which could use by intravenous injection, was not decreased compared to above three compounds by addition of silkworm hemolymph and fluconazole showed good therapeutic effect on silkworm model These results suggest that therapeutic effect of antifungals could be evaluated using silkworms, which reflected pharmacokinetics as mammals.
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Research Products
(13 results)
