2013 Fiscal Year Final Research Report
Development of fluorescent probes for metallo-beta-lactamase-producing Gram-negative bacteria
| Project/Area Number |
24659054
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Environmental pharmacy
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Yoshihiro 熊本大学, 環境安全センター, 准教授 (10363524)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 感染症 / 抗生物質 / 院内感染 / 酵素 / ラクタマーゼ / βーラクタム剤 |
|---|
| Research Abstract |
Metallo-beta-lactamase can hydrolyze nearly almost beta-lactams, including carbapenems. It is very important to confirm the production of metallo-beta-lactamase in infectious bacteria for effective chemotherapy at the initial stages of a disease. A new fluorogenic probe based on modified cephalosporin was synthesized and examined the ability of the fluorogenic probe to detect metallo-beta-lactamase (IMP-1) by measurement of the fluorescence spectra. As the concentration of IMP-1, the fluorescence intensity of the fluorogenic probe increased. Thus, the spectral data supports the validity of our design strategy. This fluorogenic probe will serve as lead compounds for the development of detection agents that are selective metallo-beta-lactamases-producing infectious bacteria.
|
