Search for intracellular introduction method of anti-tumor substance using microbial infection receptor nucleolin.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659057
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental pharmacy
• Research Institution: Takasaki University of Health and Welfare (2014); Tokyo University of Pharmacy and Life Science (2012-2013)
• Principal Investigator: HIRANO KAZUYA 高崎健康福祉大学, 薬学部, 教授 (80251221)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ヌクレオリン / ツーハイブリッドシステム / ヌクレオリン結合分子 / AGE / βアミロイド

Outline of Final Research Achievements

We found found HABP4, NUDT5, HMGB1 as a new nucleolin binding molecule using Yeast two-hybrid technique. Surface plasmon-resonance analysis revealed that these molecules had a strong binding to rNUC284.Advanced glycosylation end-products (AGEs) are non-enzymatically glycosylated proteins that play an important role in several diseases and aging processes. Surface plasmon-resonance analysis revealed that nucleolin strongly associated with GCA-BSA and GOA-BSA.We found nucleolin is a receptor that allows macrophages to recognize toxic AGEs.Amyloid-beta peptide 1-42 (Aβ42) plays a key role in the neurotoxicity found in Alzheimer's disease. We found monomeric and fibril Aβ42 were phagocytosed by mouse microglial EOC2 cells. These results indicate that nucleolin is a receptor that allows microglia to recognize monomeric and fibril Aβ42.

Free Research Field

衛生化学、生化学