2013 Fiscal Year Final Research Report
Biomarkers for ischemia and renal faiure
Project/Area Number |
24659062
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Medical pharmacy
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Research Institution | Tohoku University |
Principal Investigator |
ABE Takaaki 東北大学, 医工学研究科, 教授 (80292209)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Keywords | 1-メチルアデノシン / 修飾核酸 / 急性虚血 / 腎機能障害 / 酸化ストレス |
Research Abstract |
Tissue damage by oxidative stress is a key pathogenic mechanism in various diseases including acute kidney injury and chronic kidney disease. Its early detection is important. Using a tRNA-specific modified nucleoside 1-methyladenosine (m1A) antibody, we demonstrate oxidative stress induces direct conformational change in tRNA structure and the change promotes subsequent tRNA fragmentation. Such tRNA changes occur much earlier than DNA damage. By various types of tissue damage model (ischemic reperfusion, toxic injury), the circulating tRNA-derivative levels are increased and the increase is more rapid than the known damage markers. In humans, the circulating tRNA-derivatives also increase under renal ischemia conditions. It also correlates with mortality in the general population (n=1,033 for 6.7 year follow up) . Therefore, tRNA damage reflects early oxidative stress damage and its detection is a useful tool for identifying organ damages and forming a clinical prognosis.
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[Journal Article] Indoxyl sulfate down-regulates SLCO4C1 transporter through up-regulation of GATA2013
Author(s)
Akiyama Y., Kikuchi K., Saigusa D., Suzuki T., Takeuchi Y., Mishima E., Yamamoto Y., Ishida A., Sugawara D., Shima H., Toyohara T., Suzuki C., Souma T., Moriguchi T., Tomioka Y., Ito S. and AbeT.
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Journal Title
Plos One
Volume: 8
Pages: e66518
DOI
Peer Reviewed
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[Journal Article] Mutation of the Mg^<2+> Transporter SLC41A1 Results in a Nephronophthisis-Like Phenotype2013
Author(s)
Hurd TW, Edgar A. Otto EA, Mishima E, Gee HY, Inoue H., Inazu M., Yamada H., Halbritter J., Seki, G., Konishi M., Zhou W., Yamane T., Murakami S., Caridi G., Ghiggeri G., AbeT. and Hildebrandt F.
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Journal Title
J . Am. Soc. Nephrol.
Volume: 24
Pages: 967-977
DOI
Peer Reviewed
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