Quantitative targeted absolute proteomics-based diagnostics for personalized cancer chemotherapy

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659063
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: TERASAKI TETSUYA 東北大学, 薬学研究科(研究院), 教授 (60155463)
• Co-Investigator(Kenkyū-buntansha): UCHIDA Yasuo 東北大学, 大学院薬学研究科, 助教 (70583590) ; NAKADA Mitsutoshi 金沢大学, 医学系, 助教 (20334774) ; TACHIKAWA Masanori 東北大学, 大学院薬学研究科, 准教授 (00401810)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 分子標的薬 / 定量プロテオミクス / リン酸化 / 増殖因子受容体 / 個別化医療 / がん

Research Abstract

There has been increasing demands for molecular-targeted drugs-based personalized cancer chemotherapy. Our findings provide the basis that the characteristics of human malignant brain tumors can be stratified according to the absolute protein expression levels of target receptors. Simultaneous and highly sensitive quantitative targeted phospho-proteomics has also been established.

Research Products

• [Journal Article] Identification of transporters associated with etoposide sensitivity of stomach cancer cell lines and methotrexate sensitivity of breast cancer cell lines by quantitative targeted absolute proteomics (2013)
  • Author(s): Obuchi W, Ohtsuki S, Uchida Y, Ohmine K, Yamori T, Terasaki T
  • Journal Title: Mol Pharmacol Volume: 83(2) Pages: 490-500
  • Peer Reviewed
• [Journal Article] Quantitative Expression of Human Drug Transporter Proteins in Lung Tissues : Analysis of Regional, Gender and Inter-individual differences by Liquid Chromatography-Tandem Mass Spectrometry. E-pub ahead of print (2013)
  • Author(s): Sakamoto A, Matsumaru T, Yamamura N, Uchida Y, Tachikawa M, Ohtsuki S, Terasaki T
  • Journal Title: J Pharm Sci Volume: 102(9) Pages: 3395-3406
  • Peer Reviewed
• [Journal Article] A Study Protocol for Quantitative Targeted Absolute Proteomics (QTAP) by LC-MS/MS : Application for Inter-Strain Differences in Protein Expression Levels of Transporters, Receptors, Claudin-5, and Marker Proteins at the Blood-Brain Barrier in ddY, FVB, and C57BL/6J mice (2013)
  • Author(s): Uchida Y, Tachikawa M, Obuchi W, Hoshi Y, Tomioka Y, Ohtsuki S, Terasaki T
  • Journal Title: Fluids and Barriers of the CNS Volume: 10(1) Pages: 21
  • Peer Reviewed
• [Journal Article] 定量的標的プロテオミクスに基づく胃癌・乳癌薬剤感受性関連トランスポーターの解明 (2013)
  • Author(s): 小渕航、寺崎哲也
  • Journal Title: 医学のあゆみ Volume: 245(1) Pages: 135-141
• [Presentation] 標的絶対定量プロテオミクスに基づくがん分子標的化学療法の個別・層別化基盤の構築 (2014)
  • Author(s): 内田康雄、立川正憲、小渕航、大槻純男、中田光俊、濱田潤一郎、寺崎哲也
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-29
• [Presentation] 血液脳関門—白血球細胞遊走に関わる接着分子の標的定量プロテオミクス解析 (2014)
  • Author(s): 佐藤和貴、立川正憲、内田康雄、大槻純男、寺崎哲也
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-29
• [Presentation] 前処理自動化と質量分析を組み合わせたハイスループットMRM法の開発 (2014)
  • Author(s): 米山敏広、大槻純男、尾野雅哉、大峰健、内田康雄、立川正憲、寺崎哲也
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-29
• [Presentation] Pharmacoproteomics : Quantitative Targeted Absolute Proteomics (QTAP) based Pharmacokinetics as A New Path to the Drug Discovery and Development and the Personalized Medicine (2013)
  • Author(s): Terasaki T
  • Organizer: International Symposium for the retirement of Professor Chang Koo Shim
  • Place of Presentation: Seoul National University, Seoul, South Korea
  • Year and Date: 20130710-11
• [Presentation] 定量的標的絶対プロテオミクスに基づく癌化学療法の新展開 (2013)
  • Author(s): 寺崎哲也
  • Organizer: 公益社団法人・日本薬剤学会主催APSTJ WG 2025シンポジウム「バイオとナノが拓く医療イノベーション」
  • Place of Presentation: 東京
  • Year and Date: 2013-12-03
• [Presentation] Quantitative targeted absolute proteomics of the malignant brain tumors towards molecular-targeted chemotherapy (2013)
  • Author(s): Terasaki T, Obuchi W, Ohtsuki S, Nakada M, Uchida Y, Tachikawa M, Hamada J
  • Organizer: 10th International Society for the Study of Xenobiotics (ISSX) Meeting
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2013-09-30
• [Presentation] Pharmacoproteomics : 定量的標的絶対プロテオミクスに基づく薬剤学の新展開 (2013)
  • Author(s): 寺崎哲也
  • Organizer: 薬剤学教科担当教員会議
  • Place of Presentation: 富山
  • Year and Date: 2013-08-30
• [Presentation] 膜タンパク質を標的とした定量的標的プロテオミクスに基づく悪性脳腫瘍に対する個別化分子標的療法 (2013)
  • Author(s): 小渕航、大槻純男、中田光俊、内田康雄、立川正憲、濱田潤一郎、寺崎哲也
  • Organizer: 日本薬剤学会第28年会
  • Place of Presentation: 名古屋
  • Year and Date: 2013-05-23
• [Presentation] 膵癌細胞のゲムシタビン感受性増強を目的とした併用薬のスクリーニング (2013)
  • Author(s): 黒田広樹、立川正憲、内田康雄、寺崎哲也
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-28
• [Presentation] 仮想selective reaction monitoring法を用いた白血球細胞膜上タンパク質の高感度標的プロテオミクス (2013)
  • Author(s): 佐藤和貴、立川正憲、内田康雄、大槻純男、寺崎哲也
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-28
• [Presentation] Targeted quantitative LC-MS/MS methods of bevacizumab, a therapeutic monoclonal antibody (2012)
  • Author(s): Ebata Y, Ohtsuki S, Tachikawa M, Uchida Y, Terasaki T
  • Organizer: 日本薬物動態学会第27年会
  • Place of Presentation: 千葉
  • Year and Date: 2012-11-21
• [Presentation] Proteomics-based technology as a new path to toxicological evaluation (2012)
  • Author(s): Tachikawa M
  • Organizer: 2012 International Symposium on Green Toxicology & Technology : Prediction of Kidney Toxicity
  • Place of Presentation: Seoul, South Korea
  • Year and Date: 2012-09-07
• [Book] Blood-brain barrier (BBB) pharmacoproteomics : a new research field opened up by quantitative targeted absolute proteomics (QTAP). Drug Delivery to the Brain–Physiological Concepts, Methodologies and Approaches, Hammarlund-Udenaes M, de Lange E, and Thorne R (Ed) (2014)
  • Author(s): Uchida Y, Tachikawa M, Ohtsuki S, Terasaki T
  • Total Pages: 66-100
  • Publisher: Springer, New York