Studies on the neuron-specific transcriptional regulator Dpf1 using transgenic zebrafish expressing neuronal tracers

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659085
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kobe University
• Principal Investigator: KIKKAWA Satoshi 神戸大学, 医学(系)研究科(研究院), 准教授 (90244681)
• Co-Investigator(Renkei-kenkyūsha): TERASHIMA Toshio 神戸大学, 大学院医学研究科, 教授 (20101892)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: クロマチン再構成複合体 / 神経細胞分化

Outline of Final Research Achievements

We indentified a main dpf1 transcrypt, dpf1-002, in the zebrafish central nervous system and determined the nucleotide sequence and expression pattern in embryos. In addition, in the dpf1-tTA-GFP fish, the GFP transgene was transcrived from the start point different from that of dpf1-002, and the expression of endogenous dpf1-002 was not affected by the expression of the transgene. We intend functional inhibition using morpholino antisense oligonucleotides (MO) for dpf1-002, but unfortunately have not yet observed clear-cut developmental nerve defects in the injected embyros. Examination such as the coinjection of different MOs is necessary. The different gene targeting approach such ad genome editing may also be needed in future.

Free Research Field

神経解剖学