Analyses of a novel membrane domain involved in the survival strategy for cancer cell

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659088
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Fukushima Medical University
• Principal Investigator: WAGURI Satoshi 福島県立医科大学, 医学部, 教授 (30244908)
• Co-Investigator(Kenkyū-buntansha): UEMURA Takefumi 福島県立医科大学, 医学部, 講師 (80548925)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Dipeptidase1 / 癌細胞 / 膜ドメイン / 酸化ストレス

Outline of Final Research Achievements

Cellular localization and functions of dipeptidase 1 (DPEP1), a marker of colorectal cancer, were investigated in this study. In a HCC56 cell line that expresses DPEP1 at high levels, DPEP1 was found to be localized on plasma membrane domains containing GPI-anchored protein, and at the bottom of microvilli. Suppression of DPEP1 by RNAi experiment did not affect the cell growth, but caused a vulnerability to oxidative stress, suggesting that DPEP1 might be involved in the tolerance of cancer cells against oxidative stress.　Therefore, this study proposes a new mechanism involving DPEP1, which could be a target of anticancer drug development.

Free Research Field

細胞生物学、細胞組織学