2014 Fiscal Year Final Research Report
Preparation of insulin-producing cells using the parafollicular cells and attempt to improve insulin secretion in diabetic mice
| Project/Area Number |
24659090
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 傍濾胞細胞 / カルシトニン / インスリン / 膵β細胞 / 再生 / 分化 |
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| Outline of Final Research Achievements |
The author discovered that parafollicular cells synthesized insulin to the early stage of the development. Based on this finding, parafollicular cells were examined whether can differentiate into pancreatic β cells. Genes related to differentiation of pancreatic β cells were introduced into cultured parafollicular cells at the early stage of the development. Insulin gene expression were increased 19-fold by introducing MafA and the Pdx1 gene simultaneously. The cultured cells were confirmed to produce insulin by immunostaining. The present result suggested that parafollicular cells is capable of regenerating pancreatic β cells.
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| Free Research Field |
分子内分泌
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