2015 Fiscal Year Final Research Report
The establishment of anterograde tracing label of vagus nerve terminal in mice.-Resolution of the onset of hiding deabetes-
| Project/Area Number |
24659091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 神経標識 |
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| Outline of Final Research Achievements |
The goal the present study is to clarify the onset mechanism of the hiding diabetes. Melanocortin-4 receptor(MC4-R) has been noticed as critical regulator of insulin secretion. Morphological and histochemical analysis on MC4-R GFP mice indicates that the possibilities that the DMV neurons directly project to B cell in the pancreas. The method to efficiently label the vagus nerve terminals with anterograde tracer has been examined. The interesting combination of the tracer conjugated with special fluorescence agent and high sensible detector “IVIS Imaging system” may produce not only time-saving of the work but also decrease of a number of mice sacrified has been found. The working condition of the tracer is problem to be solved in the future.
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| Free Research Field |
組織学
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