Towards better understanding of the molecular mechanisms of functional diversity of the electrogenic sodium bicarbonate cotransporter

2015 Fiscal Year Final Research Report

• Project/Area Number: 24659094
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Ishikawa Toru 帯広畜産大学, 畜産学部, 教授 (70249960)
• Research Collaborator: KOTERA Akira 帯広畜産大学, 畜産学部, 学生 ; KAMADA Takuro 帯広畜産大学, 畜産学部, 学生 ; NII Takeshi 帯広畜産大学, 畜産学部, 学生
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 起電性ナトリウム重炭酸イオン共輸送体 / pH / マグネシウムイオン / カリウムチャネル

Outline of Final Research Achievements

The main aim of this project was to gain some clues as to the cellular and molecular mechanisms of functional diversity of the electrogenic sodium bicarbonate cotransporter NBCe1-B observed in various native tissues and cell types. Using a heterologous mammalian expression system, H+ was suggested to be a cytosolic factor that might modify the intracellular Mg2+-sensitivity of NBCe1-B. Studies on ion channels in bovine parotid acinar cells endogenously expressing NBCe1-B may raise the possibilities that pH-sensitive K+ currents and TRPM6/7-like cation channel activity might be involved, at least in part, in regulation of NBCe1-B in these cells. The data obtained in this project will be useful for future studies to better understand regulatory mechanisms of the electrogenic sodium bicarbonate cotransporter activity in intact cells.

Free Research Field

細胞生理学