2013 Fiscal Year Final Research Report
Elucidation of the unidentified molecular structure of the cell volume-sensitive anion channel
| Project/Area Number |
24659095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | University of Toyama |
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Principal Investigator |
SAKAI Hideki 富山大学, 大学院医学薬学研究部(薬学), 教授 (60242509)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Takahiro 富山大学, 大学院医学薬学研究部(薬学), 准教授 (40353437)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 生理学 / 細胞・組織 / 蛋白質 / イオンチャネル / 細胞容積 / 浸透圧 |
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| Research Abstract |
Even if almost all the cells swell under hypotonic condition, they can return to their original sizes. This is because the cell has housekeeping functions for regulating its volume. In the mechanism, the cell volume-sensitive anion channel (VSOR) is one of essential proteins. Molecular structure of VSOR, however, has not been identified yet. Here, we synthesized the photoaffinity probe based on the structure of DCPIB, a specific inhibitor of VSOR. In a series of experiments such as the analysis of labelled proteins and the electrophysiological patch-clamp recording, we found that VC-1 (tentative name) is an essential molecule which constitutes VSOR.
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Research Products
(32 results)
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[Journal Article] Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells2014
Author(s)
Ikari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J
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Journal Title
J. Biol. Chem.
Volume: 289
Pages: 13112-13123
DOI
Peer Reviewed
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[Journal Article] Functional coupling of chloride-proton exchanger ClC-5 to gastric H^+,K^+-ATPase2014
Author(s)
Takahashi Y, Fujii T, Fujita K, Shimizu T, Higuchi T, Tabuchi Y, Sakamoto H,Naito I, Manabe K, Uchida S, Sasaki S, Ikari A, Tsukada K, Sakai H
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Journal Title
Biol. Open
Volume: 3
Pages: 12-21
DOI
Peer Reviewed
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[Journal Article]2012
Author(s)
Nishi M, Aoyama F, Kisa F, Zhu H, Sun M, Lin P, Ohta H, Van B, Yamamoto S, Kakizawa S, Sakai H, Ma J, Sawaguchi A, Takeshima H
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Journal Title
J. Biol. Chem.
Volume: 287
Pages: 33523-33532
DOI
Peer Reviewed
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