Single-cell recording of suprachiasmatic nucleus in rodent

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659109
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YAGITA Kazuhiro 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90324920)
• Co-Investigator(Kenkyū-buntansha): INOKAWA Hitoshi 京都府立医科大学, 医学研究科, 講師 (40285250)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 概日リズム / 視交叉上核 / 単一ニューロン / 電気活動

Research Abstract

Single-cell based neural activity recording from the suprachismatic nucleus is important to understand the resetting mechanism of circadian rhythm in mammals. We performed single-cell neural activity recording from living rat or mouse suprachiasmatic nucleus. We succeeded to record the single-cell neural activity responded to the light in mice and rats. It must be useful strategy to elucidate the resetting mechanisms in mammalian circadian clock.

Research Products

• [Journal Article] Prolonged Bioluminescence Monitoring in Mouse ex vivo Bone Culture Revealed Persistent Circadian Rhythms in Articular Cartilages and Growth Plates (2013)
  • Author(s): Okubo N, Minami Y, Fujiwara H, Umemura Y, Tsuchiya Y, Shirai Y, Oda R, Inokawa H, Kubo T, Yagita K
  • Journal Title: PLoS One Volume: 8 Pages: e78306
  • Peer Reviewed
• [Journal Article] Regional circadian period difference in the suprachiasmatic nucleus of the mammalian circadian center (2013)
  • Author(s): Koinuma S, Asakawa T, Nagano M, Furukawa K, Sujino M, Masumoto KH, Nakajima Y, Hashimoto S, Yagita K, Shigeyoshi Y
  • Journal Title: Eur J Neurosci Volume: 38 Pages: 2832-41
  • Peer Reviewed
• [Journal Article] An in vitro ES cell-based clock recapitulation assay model identifies CK2a as an endogenous clock regulator (2013)
  • Author(s): Umemura Y, Yoshida J, Wada M, Tsuchiya Y, Minami Y, Watanabe H, Kondoh G, Takeda J, Inokawa H, Horie K, Yagita K
  • Journal Title: PLoS One Volume: 8 Pages: e67241
  • Peer Reviewed
• [Presentation] Misregulation of transcriptional program controlling the cellular differentiation resulted in disruption of the circadian clock development (2014)
  • Author(s): 八木田和弘(シンポジスト・オーガナイザー)
  • Organizer: 日本生理学会大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2014-03-16
• [Presentation] Circadian Clock : A cell-autonomous time-keeping system in our body (2013)
  • Author(s): Yagita K
  • Organizer: 6^<th> Asia Oceania Conference of Photobiology
  • Place of Presentation: Sydney(Invited speaker)
  • Year and Date: 20130000
• [Presentation] ES cell-based in vitro evaluation system of circadian clock phenotypes in mammals (2013)
  • Author(s): 八木田和弘(シンポジスト・オーガナイザー)
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20130000
• [Presentation] MYC induced disruption of circadian clock development (2013)
  • Author(s): 八木田和弘(シンポジスト・オーガナイザー)
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-12-11
• [Presentation] イメージング技術を用いた生体機能のCutting Edge (2013)
  • Author(s): 八木田和弘
  • Organizer: 関西実験動物研究会30周年記念大会
  • Place of Presentation: 京都(シンポジスト)
  • Year and Date: 2013-12-06
• [Presentation] ES細胞を用いた概日時計のin vitro再現と分化制御機構との関連 (2013)
  • Author(s): 八木田和弘(オーガナイザー)
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸(ワークショップ講演)
  • Year and Date: 2013-12-05
• [Presentation] 体内時計はいつ動き出すか (2013)
  • Author(s): 八木田和弘(シンポジスト・オーガナイザー)
  • Organizer: 第14回日本抗加齢医学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-06-30
• [Presentation] ES cell-based approach elucidating the integrated physiology of mammalian circadian systems (2012)
  • Author(s): Yagita K
  • Organizer: 4th International Symposium on Photic Bioimaging 2012
  • Place of Presentation: Sapporo(Invited speaker)
  • Year and Date: 20120000
• [Presentation] Cell-autonomous development of mammalian circadian clock during the differentiation culture of ES cells (2012)
  • Author(s): Yagita K
  • Organizer: International Conference for Histochemistry and Cytochemistry 2012
  • Place of Presentation: Kyoto(Invited speaker)
  • Year and Date: 20120000
• [Presentation] 体内時計の発生・発達 (2012)
  • Author(s): 八木田和弘
  • Organizer: 第1回日本発達神経科学会
  • Place of Presentation: 明石(特別講演)
  • Year and Date: 20120000
• [Presentation] 発生発達期における概日リズムの階層的形成 (2012)
  • Author(s): 八木田和弘
  • Organizer: 第17回日本行動神経内分泌研究会
  • Place of Presentation: 京都(教育講演)
  • Year and Date: 20120000
• [Presentation] 時間軸生物学 : 細胞分化と概日時計の接点 (2012)
  • Author(s): 八木田和弘(シンポジスト・オーガナイザー)
  • Organizer: 第12回日本抗加齢医学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20120000