2013 Fiscal Year Final Research Report
A new method to sub-fractionate hematopoietic stem cells based on signal response characteristics
| Project/Area Number |
24659127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OTSU MAKOTO 東京大学, 医科学研究所, 准教授 (30361330)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Yoshiki 九州大学, 工学(系)研究科(研究院), 教授 (70284528)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 造血幹細胞 / シグナル伝達 |
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| Research Abstract |
In this project we have aimed at the development of a new method to isolate previously unidentified subfractions in hematopoietic stem cells (HSC) by utilizing their varying responsiveness to a certain cytokine signal. Intracellular markers have been developed as the fluorescent nanoparticles containing peptide substrates for certain kinases. After screening candidate peptide substrates, the synthesized marker will be introduced into HSC to detect and/or subfractionate them for characterization.
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Research Products
(15 results)
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[Journal Article] Stage-specific roles for Cxcr4 signaling in murine hematopoietic stem/progenitor cells in the process of bone marrow repopulation2014
Author(s)
Lai, C. Y., Yamazaki, S., Okabe, M., Suzuki, S., Maeyama, Y., Iimura, Y., Onodera, M., Kakuta, S., Iwakura, Y., Nojima, M., Otsu, M., & Nakauchi, H.
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Stabilization of cancer-specific gene carrier via hydrophobic interaction for a clear-cut response to cancer signaling2013
Author(s)
Kim, C. W., Toita, R., Kang, J. H., Li, K., Lee, E. K., Zhao, G. X., Funamoto, D., Nobori, T., Nakamura, Y., Mori, T., Niidome, T., & Katayama, Y.
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Journal Title
J Control Release
Volume: 170(3)
Pages: 469-476
DOI
Peer Reviewed
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[Journal Article] Top-down motif engineering of a cytokine receptor for directing ex vivo expansion of hematopoietic stem cells2013
Author(s)
Saka, K., Kawahara, M., Teng, J., Otsu, M., Nakauchi, H., & Nagamune, T.
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Journal Title
J Biotechnol
Volume: 168(4)
Pages: 659-665
DOI
Peer Reviewed
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[Journal Article] A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides2013
Author(s)
Shiosaki, S., Nobori, T., Mori, T., Toita, R., Nakamura, Y., Kim, C. W., Yamamoto, T., Niidome, T., & Katayama, Y.
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Journal Title
Chem Commun (Camb)
Volume: 49(49)
Pages: 5592-5594
DOI
Peer Reviewed
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[Journal Article] Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation2013
Author(s)
Suzuki, N., Yamazaki, S., Yamaguchi, T., Okabe, M., Masaki, H., Takaki, S., Otsu, M., & Nakauchi, H.
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Journal Title
Mol Ther
Volume: 21(7)
Pages: 1424-1431
DOI
Peer Reviewed
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[Journal Article] Generation of induced pluripotent stem cells from primary chronic myelogenous leukemia patient samples2012
Author(s)
Kumano, K., Arai, S., Hosoi, M., Taoka, K., Takayama, N., Otsu, M., Nagae, G., Ueda, K., Nakazaki, K., Kamikubo, Y., Eto, K., Aburatani, H., Nakauchi, H., & Kurokawa, M.
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Journal Title
Blood
Volume: 119(26)
Pages: 6234-6242
DOI
Peer Reviewed
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