2013 Fiscal Year Final Research Report
Study on the mechanism of tissue specific diseases caused by mRNA splicing defect
| Project/Area Number |
24659129
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | University of Toyama |
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Principal Investigator |
KAIDA DAISUKE 富山大学, 先端ライフサイエンス拠点, 特命助教 (60415122)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | mRNAスプライシング / 組織特異的疾患 |
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| Research Abstract |
We treated mammalian cells with antisense oligo for each component of spliceosome and found that all antisense oligo could inhibit splicing activity in a dose-dependent manner. Splicing inhibition by the antisense oligos decreased cell survival rate and interestingly the magnitude of the effect was different among cell lines.
We also treated mouse 3T3-L1 cells with a splicing inhibitor and found that adipogenesis was defective after splicing inhibitor treatment. This inhibition was also affected the timing of addition of splicing inhibitor.
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