Exploration of novel molecular targets for anti-cancer drug in long non-coding RNAs

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: KITAGAWA Masatoshi 浜松医科大学, 医学部, 教授 (50294971)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ノンコーディングRNA / 癌抑制遺伝子 / INK4 locus / エピジェネティクス

Research Abstract

lncRNA ANRIL negatively regulates tumore suppressor p16Ink4 via recruitment of PRC2. We found that RNA immunoprecipitation method is applicable for a screening system to evaluate the binding between ANRIL and SUZ12, a component of PRC2. Moreover, we developed quantitation methods to measure fifteen known lncRNAs including ANRIL to evaluate their association with cancer development. Although, we evaluate them in hepatocellular carcinoma cell lines with or without HBx-expression using this system, there are no lncRNA oscillating by HBx-expression. Further evaluation will be required.

Research Products

• [Journal Article] YB-1 promotes transcription of cyclin D1 in human non-small-cell lung cancers (2014)
  • Author(s): Harada, M., Kotake, Y., Ohhata, T., Kitagawa, K., Niida, H., Matsuura, S., Funai, K., Sugimura, H., Suda, T. and Kitagawa, M.
  • Journal Title: Genes Cells
  • DOI: 10.1111/gtc.12150
• [Journal Article] Interaction between RB protein and NuMA is required for proper alignment of spindle microtubules (2014)
  • Author(s): Uchida, C., Hattori, T., Takahashi, H., Yamamoto, N., Kitagawa, M. and Taya, Y.
  • Journal Title: Genes Cells Volume: 19 Pages: 89-96
  • DOI: 10.1111/gtc.12119
• [Journal Article] Anti-VEGF antibody therapy induces tumor hypoxia and stanniocalcin 2 expression and potentiates growth of human colon cancer xenografts (2014)
  • Author(s): Miyazaki, S., Kikuchi, H., Iino I., Uehara, T, Setoguchi, T., Fujita, T., Hiramatsu, Y., Ohta, M., Kamiya, K., Kitagawa, K., Kitagawa, M., Baba, S. and Konno, K.
  • Journal Title: Int. J. Cancer Volume: 135 Pages: 295-307
  • DOI: 10.1002/ijc.28686
• [Journal Article] Cell cycle regulation by long non-coding RNAs (2013)
  • Author(s): Kitagawa, M., Kitagawa, K., Kotake, Y., Niida, H. and Ohhata, T.
  • Journal Title: Cell. Mol. Life Sci. Volume: 70 Pages: 4785-4794
  • DOI: 10.1007/s00018-013-1423-0
• [Journal Article] YB1 binds to and represses the p16 tumor suppressor gene (2013)
  • Author(s): Kotake, Y., Ozawa, Y., Harada, M., Kitagawa, K., Niida, H., Morita, Y., Tanaka, K., Suda, T. and Kitagawa, M.
  • Journal Title: Genes Cells Volume: 18 Pages: 999-1006
  • DOI: 10.1111/gtc.12093
• [Journal Article] Roles of the Skp2/p27 axis in the progression of chronic nephropathy (2013)
  • Author(s): Suzuki, S., Ohashi, N. and Kitagawa, M.
  • Journal Title: Cell. Mol. Life Sci. Volume: 70 Pages: 3277-3289
  • DOI: 10.1007/s00018-012-1232-x
• [Journal Article] Long non-coding RNAs in cancer development and cell fate determination (2012)
  • Author(s): Kitagawa, M., Kotake, Y. and Ohhata, T.
  • Journal Title: Cell. Curr. Drug Targets Volume: 13 Pages: 1616-1621
  • DOI: 10.2174/138945012803530026
• [Presentation] マウスES細胞におけるXist抑制機構解析 (2013)
  • Author(s): 大畑樹也、松本美香、Martin Leeb,柴田進和、廣瀬哲郎、北川恭子、丹伊田浩行、北川雅敏、Anton Wutz
  • Organizer: 第36回日本分子生物学会年会ポスター発表
  • Place of Presentation: 神戸
  • Year and Date: 20130000
• [Presentation] Investigation of long non-coding RNAs involved in regulating X-chromosome inactivation (2013)
  • Author(s): Tatsuya Ohhata, Masatoshi Kitagawa
  • Organizer: The 13th Hamamatsu- Kyungpook Joint Medical Symposium, Plenary talk
  • Place of Presentation: Deagu, Korea
  • Year and Date: 20130000
• [Presentation] Molecular dissection of Xist repression through Tsix dependent/independent manner in undifferentiated ES cells (2013)
  • Author(s): Tatsuya Ohhata, Mika Matsumoto, Martin Leeb, Shinwa Shibata, Tesuro Hirose, Masatoshi Kitatgawa, Anton Wutz
  • Organizer: Gordon research conference, Epigenetics
  • Place of Presentation: Rhode Island, USA
  • Year and Date: 20130000
• [Presentation] Recent progress of Long non-coding RNAs research (2012)
  • Author(s): 大畑樹也、北川雅敏
  • Organizer: 第71回日本癌学会年会シンポジウム発表
  • Place of Presentation: 札幌
  • Year and Date: 20120000