2012 Fiscal Year Final Research Report
Investigating the role of IKK/NF-・B signaling axis on the cellular reprogramming
| Project/Area Number |
24659133
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
KATO Tomohisa 京都大学, 再生医科学研究所, 講師 (50301247)
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| Co-Investigator(Kenkyū-buntansha) |
TOGUCHIDA Junya 京都大学, 再生医科学研究所, 教授 (40273502)
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| Co-Investigator(Renkei-kenkyūsha) |
AOI Takashi 京都大学, iPS細胞研究所, 教授 (00546997)
TAKAHASHI Kazutoshi 京都大学, iPS細胞研究所, 講師 (80432326)
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| Project Period (FY) |
2012
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| Keywords | 細胞初期化 / iPS 細胞 / シグナル伝達 / IKK / NF-κB |
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| Research Abstract |
We have investigated the role, if any, of IKK/NF-κB signaling axis in cellular reprogramming into iPS cells. We found that ectopic expr ession of Yamanaka 4 factors induced the IKKβ kinase activity depending on ATM. Moreover, disruption of Ikkβ gene reduced the reprogramming efficiency, whereas co-expr ession of IKKβ^(EE), a constitutive active form of IKKβ, with Yamanaka 4 factors promoted the efficiency. We found disruption of Ikkβ elevated the number of senescent cells during the reprogramming. Collectively, our results indicate that IKKβ potentiates cellular reprogramming by antagonizing the cellular senescence evoked by the ectopic expression of Yamanaka factors in anAT M-dependent manner.
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[Journal Article] Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin2013
Author(s)
Nasu, A., Ikeya, M., Yamamoto, T., Watanabe, A., Jin, Y., Matsumoto, Y., Hayakawa, K., Amano, N., Sato, S., Osafune, K., Aoyama, T., Nakamura, T., Kato, T. and Toguchida, J
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Journal Title
PLoS ONE
Volume: 8
Pages: e53771
DOI
Peer Reviewed
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[Journal Article] Identification of target genes of synovial sarcoma-associated fusion oncoprotein using human pluripotent stem cells2013
Author(s)
Hayakawa, K., Ikeya, M., Fukuta, M., Woltjen, K., Tamaki, S., Takahara, N.,Kato, Jr., T., Sato, S., Otsuka, T and Toguchida, J
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Journal Title
Biochem Biophys Res Commun
Volume: 432
Pages: 713-719
DOI
Peer Reviewed
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[Journal Article] The transcription factor Sp3 regulates the expression of a metastasis-related marker of sarcoma, actin filament-associated protein 1-like 1 (AFAP1L1).2013
Author(s)
Kajita, Y,Kato, Jr., T., Tamaki, S., Furu, M., Takahashi R., Nagayama, S., Aoyama, T., Nishiyama, H., Nakamura, E., Katagiri, T., Nakamura, Y., Ogawa, O. and Toguchida, J. 4.
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Journal Title
PLoS ONE
Volume: 8
Pages: e49709
DOI
Peer Reviewed
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[Journal Article] Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells2012
Author(s)
Kajiwara, M., Aoi, T., Okita, K., Takahashi, R.,Inoue, H., Takayama, N., Endo, H., Eto,K., Toguchida, J., Uemoto S, Yamanaka S
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Journal Title
Proc Natl Acad Sci USA
Volume: 109
Pages: 12538-12543
DOI
Peer Reviewed
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