2013 Fiscal Year Final Research Report
Artificial induction of DNA methylation
| Project/Area Number |
24659135
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
NAKANO Toru 大阪大学, 生命機能研究科, 教授 (00172370)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | DNAメチル化 / 遺伝子発現制御 / 生殖細胞 |
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| Research Abstract |
We planned to establish two novel strategies to introduce artificial DNA methylation using zinc finger fusion proteins and small RNAs. One is the introduction of de novo DNA methylation in embryonic male germ cells by using the fusion protein of zinc finger protein and MIWI2 protein. The other is the introduction of DNA methylation in culture cells by using the triplex nucleic acids consisting of two DNA strands and one RNA strand. Although, unfortunately, the latter experiment did not work, we succeeded in establishing the latter one. Following the success, we are currently analyzing the function of MIWI2 in the embryonic male germ cells.
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[Journal Article] GPAT2, a Mitochondrial Outer Membrane Protein in piRNA Biogenesis in Germline Stem Cells2013
Author(s)
Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
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Journal Title
RNA
Volume: 19
Pages: 803-810
DOI
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[Journal Article] Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus2013
Author(s)
Yamamoto Y,Watanabe T,Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H
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Journal Title
Genome Res
Volume: 23
Pages: 292-299
DOI
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