Development of new therapies for tumor and autoimmunity using CD8aaT cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659148
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: SUGAI Manabu 京都大学, 医学(系)研究科(研究院), 講師 (90303891)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 自己免疫疾患 / 制御性T細胞

Research Abstract

Cell fate determination of helper T cells (CD4 T cells) or cytotoxic T cells (CD8 T cells) is mutually exclusive process depending on antigen recognition, which occurs in the thymus. We found that retinoic acid and transforming growth factor-b1 promoted the differentiation of CD8 aaT cells from CD4 T cells in peripheral lymphoid tissues. And we examined the expression patterns of various genes in differentiated CD8aa T cells and compared with those in iTreg cells. Furthermore, we found various compounds, which regulate differentiation of CD8aa T cells from CD4 cells.

Research Products

• [Journal Article] Functional Evaluation of Activation-dependent Alterations in the Sialoglycan Composition of T Cells (2014)
  • Author(s): Naito-Matsui, Y., Takada, S., Kano, Y., Iyoda, T., Sugai, M., Shimizu, A., Inaba, K., Nitschke, L., Tsubata, T., Oka, S., Kozutsumi, Y. and Takematsu, H.
  • Journal Title: J . Biol. Chem. Volume: 289 Pages: 1564-1579
• [Journal Article] Phenotypes of CCAAT/enhancer-binding protein beta deficiency : hyperdontia (2013)
  • Author(s): Huang, B., Takahashi, K., Sakata-Goto, T., Kiso, H., Togo, Y., Saito, K., Tsukamoto, H., Sugai, M., Shimizu, A. and Bessho, K.
  • Journal Title: Oral Diseases Volume: 19
• [Journal Article] The metalloid arsenite induces nuclear export of Id3 possibly via binding to the N-terminal cysteine residues (2013)
  • Author(s): Kurooka, H., Sugai, M., Mori, K. and Yokota, Y.
  • Journal Title: Biochem.Biophys. Res. Commun. Volume: 433
• [Journal Article] Evasion from accelerated blood clearance of nanocarrier named as"Lactosome" (2013)
  • Author(s): Hara, E., Makino, A., Kurihara, K., Sugai, M.,Shimizu, A., Hara, I., Ozeki, E., Kimura, S.
  • Journal Title: Biochim. Biophys. Acta Volume: 1830
• [Journal Article] In situ differentiation of CD8ααT cells from CD4 T cells in peripheral lymphoid tissues (2012)
  • Author(s): Nambu, Y., Hayashi, T., Jang, KJ., Aoki, K., Mano, H., Nakano, K., Osato, M., Takahashi, K., Itoh, K., Teramukai, S., Komori, T.,Fujita, J., Ito, Y., Shimizu, A. & Sugai, M.
  • Journal Title: Scientific Reports Volume: 2 Pages: 645-623
• [Journal Article] Id2 controls chondrogenesis acting downstream of BMP signaling during maxillary morphogenesis (2012)
  • Author(s): Sakata-Goto, T., Takahashi, K., Kiso, H., Huang, B., Tsukamoto, H., Takemoto, M., Hayashi, T., Sugai, M., Nakamura, T., Tokota, Y., Shimizu, A., Slavkin, H. and Bessho, K.
  • Journal Title: Bone Volume: 50 Pages: 69-78
• [Journal Article] 制御性T細胞と大腸炎関連派発癌GI (2012)
  • Author(s): 菅井学、南部由希子、清水章
  • Journal Title: Research Volume: 20(6) Pages: 466-471
• [Presentation] Essential role of SIP1 and CD19 in B cell differentiation (2013)
  • Author(s): Hayashi T, Nambu Y, Jang KJ, Mano H, Higashi Y, Kristin V, Danny H, Shimizu A, Sugai M
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20131200
• [Presentation] Essential role of SIP1 and CD19 in B cell differentiation (2012)
  • Author(s): Hayashi T, Nambu Y, Jang KJ, Mano H, Higashi Y, Kristin V, Danny H, Shimizu A, Sugai M
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20121200
• [Presentation] In situ differentiation of CD8aa T cells from CD4 Tcells in peripheral lymphoid tissues (2012)
  • Author(s): Nambu Y, Hayashi T, Jang KJ, Aoki K, Mano H, Nakano K, Osato M, Takahashi K, Itoh K, Teramukai S, Komori T, Fujita J, Ito Y, Shimizu A, Sugai M
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Patent(Industrial Property Rights)] T細胞の分化誘導方法、T細胞の製造方法、T細胞、医薬組成物、及び、スクリーニング方法 (2013)
  • Inventor(s): 菅井学、清水章、南部由希子
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Rights Type: PTC
  • Industrial Property Number: PTC/JP2013/056758
  • Filing Date: 2013-03-12
  • Overseas
• [Patent(Industrial Property Rights)] T細胞の分化誘導方法、T細胞の製造方法、T細胞、医薬組成物、及び、スクリーニング方法 (2012)
  • Inventor(s): 菅井学、清水章、南部由希子
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Rights Type: 特願
  • Industrial Property Number: 2012-101390
  • Filing Date: 2012-04-26