2013 Fiscal Year Final Research Report
Producing the monitor mouse of alternative autophagy
| Project/Area Number |
24659178
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ARAKAWA Satoko 東京医科歯科大学, 難治疾患研究所, 助教 (90415159)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | オートファジー / モニターマウス |
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| Research Abstract |
The purpose of this research was producing the transgenic mouse for monitoring Atg5/Atg7 independent alternative autophagy. Atg5/Atg7 dependent conventional autophagy could be detected by using the GFP-LC3 expressing transgenic mouse. In contrast, the alternative autophagy could not (nature 2009).
First, we produced the transgenic mouse for visualizing autolysosome. Then we mated it with Atg5 knockout mouse and analyzed the Atg5 deficient transgenic mouse. As a result, it was indicated that alternative autophagy was involved in cardiac valve formation. Next, we generated the transgenic mouse for visualizing the Golgi apparatus because we had discovered the isolation membranes were generated from the Golgi apparatus in the alternative autophagy. Furthermore, we produced transgenic mouse that expresses the GFP tagged molecules identified as molecules that were involved in alternative autophagy.
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