2014 Fiscal Year Final Research Report
Establishment of FRET probe specific for Methylation at Histone H3 Lysine79 (H3K79me)
| Project/Area Number |
24659179
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | エピジェネティクス / Dot1L / メチル化 / 可視化 |
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| Outline of Final Research Achievements |
Methylation at lysine 79 in histone H3 (H3K79me) is known to contribute transcriptional activation, development of circular system, and differentiation of ES cells. However, no techniques to visualize H3K79me in live cells have been established yet. In this study, first, Visualization of association between a H3K79me binding protein and H3K79me by using FRET technique was challenged, but no significant results were obtained. Thus, I next searched as-yet-unknown proteins to recognize H3K79me, and identified several candidate proteins. This result will not only enables H3K79me to be visualized, but also contribute to uncover the physiological roles of H3K79me.
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| Free Research Field |
エピジェネティクス
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