Search of oxidized protein involved in the mechanism of central nervous system impairment of Down syndrome model Ts65Dn mouse

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659186
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: MOTOTADA Shichiri 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (20434780)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Toyofumi 大阪医科大学, 医学部 (10247843)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ダウン症 / 酸化ストレス / 酸化修飾蛋白質

Outline of Final Research Achievements

This study was designed to clarify the involvement of oxidative stress and oxidized proteins on Down syndrome. Overexpression of fatty acid binding protein was observed in the brain tissue of Ts65Dn mouse, a Down syndrome model mouse, by using MALDI-imaging. And the increase of oxidized DJ-1 protein in RBC of Ts65Dn mice. It was demonstrated that the open arm ratio on elevated plus maze task was increased in Ts65Dn mouse with their weeks of age. Chronic supplementation with α-tocopherol from the embryonic stage improved this open arm ratio of Ts65Dn mice. These results indicated that oxidative stress invulved in the impairment of this mouse.

Free Research Field

酸化ストレス