2013 Fiscal Year Final Research Report
Isolation of longevity related genes of prasitic nematodes with east epression system
| Project/Area Number |
24659190
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 線虫 / 寄生 / 寿命 / ゲノム |
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| Research Abstract |
Parasitic animals live longer as compared with related free-living species. In order to elucidate molecular basis for the longevity, we constructed a high-quality draft genome of an intestinal nematode, Strongyloides venezuelensis, to approach this issue with bioinformatics. Statistics of our draft genome were as follows; total size 55.5 Mb, longest scaffold 15Mb, N50 scaffold length 2Mb, with 18,048 predicted coding genes.
One of the genes which exist only in parasitic nematodes but not in free-living nematodes was that for ferrochelatase, which catalyzes the final step of hem-biosynthesis. We confirmed that this gene of S. venezuelensis was perfectly functional, though all nematodes including animal parasitic nematodes do not synthesize hem by themselves. Phylogenetic analysis suggested that animal parasitic nematodes acquired this gene from bacteria through horizontal gene transfer. The physiological role could be to detoxify hem, which is highly toxic to cells.
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[Journal Article] Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads
Author(s)
Kajitani R, Toshimoto K, Noguchi H, Toyoda A, Ogura Y, Okuno M, Yabana M, Harada M, Nagayasu E, Maruyama H, Hayashi T, Kohara Y, Fujiyama A, Itoh T.
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Journal Title
Genome. Res
Volume: (in press)
Peer Reviewed
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