T4SS supermolecular complex containing core complex and ATPase

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659198
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: NAGAI Hiroki 大阪大学, 微生物病研究所, 准教授 (80222173)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細菌 / タンパク質分泌 / IV型分泌 / レジオネラ / 接合伝達

Outline of Final Research Achievements

Bacterial pathogens manipulate host cellular processes using their own proteins. These proteins, so-called effectors, are injected into host cells using specialized nanomachines assembled　on the bacterial cell surface. One such nanomachine is the type IV secretion system (T4SS). we have successfully showed the electron microscopic structure of the core complex of the Legionella Dot/Icm T4SS. Here we report in vivo evidence for a larger complex containing the core complex, DotI-DotJ inner-membrane complex and ATPase DotO, which is essential for Legionella pathogenesis.

Free Research Field

医歯薬学