2012 Fiscal Year Final Research Report
Development ofthe infection and proliferation systemof the 1bgenotype HCV based on the new idea
| Project/Area Number |
24659207
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Okayama University |
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Principal Investigator |
KATO Nobuyuki 岡山大学, 大学院・医歯薬学総合研究科, 教授 (40150883)
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| Co-Investigator(Kenkyū-buntansha) |
DANSAKO Hiromichi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (80379841)
MORI Kyoko 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10633604)
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| Project Period (FY) |
2012
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| Keywords | C型肝炎ウイルス / 遺伝子型1b / 感染増殖システム / HCV RNA複製細胞 / 宿主因子 |
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| Research Abstract |
We tried the development of the infection and proliferation system of the 1b genotype hepatitis C virus (HCV) using human cultured cell lines. In this study, HCV-positive serum (1b genotype) was added tothe human hepatoma cell line Li23, which was found in 2009 as a cell line permitting the reproduction of the HCV RNA and human hepatoma cell line HuH-7 frequently used all over the world. We performed HCV infection under various conditions, however, we did not succeed to demonstrate an increase of the HCV. As one of the causes, we found that expression level of CLDN1, which was one of the HCV receptors, became very low, and thatthe supplement of CLDN1 expression was necessary.
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