2013 Fiscal Year Final Research Report
Molecular modification for MHC class I and II antigen presentation
| Project/Area Number |
24659211
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Kitasato University |
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Principal Investigator |
MORIKAWA Yuko 北里大学, 大学院感染制御科学府, 教授 (20191017)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 分子修飾 / 抗原提示 |
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| Research Abstract |
HIV-1 Gag protein was expressed in HeLa cells and influenza virus HA in MDCK cells. When the protein degradation kinetics were analyzed by Western blotting, we found the t1/2 of Gag 8-9 hrs and that of HA slightly shorter. Gag and HA were more rapidly degradated by the proteasome pathway, when ubiquitin was added to their N-termini. Based on the N-end rule, when the N-terminal methionine of Gag after ubquitin cleavage was substituted with arginine, the Gag was more efficiently degradated by the ubquitin/proteasome pathway. In contrast, the addition of LAMP1 to Gag altered the major degradation pathway for Gag from the proteasome pathway to the lysosomal pathway.
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[Journal Article] Sensitive detection of measles virus infection in the blood and tissues of humanized mouse by one-step quantitative RT-PCR2013
Author(s)
Ikeno S, Suzuki M, Mahmod M, Ishige M, Kobayashi M, Ohno S, Takeda M, Nakayama T, Morikawa Y, Terahara K, Okada S, Takeyama H, Tsunetsugu-Yokota Y.
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Journal Title
Front Microbiol
Volume: 4
Pages: 1-8
DOI
Peer Reviewed
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[Journal Article] HIV-1 infection ex vivo accelerates measles virus infection by upregulating signaling lymphocytic activation molecule (SLAM) in CD4+ T cells2012
Author(s)
Mitsuki Y, Terahara, K, Shibusawa K, Yamamoto T, Tsuchiya T, Ishige, M, Kobayashi K, Morikawa Y, Nakayama T, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y.
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Journal Title
J Virol
Volume: 86(13)
Pages: 7227-7234
DOI
Peer Reviewed
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