2013 Fiscal Year Final Research Report
Architecture and Function of the Epstein-Barr virus Replication Compartment
| Project/Area Number |
24659213
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | 愛知県がんセンター(研究所) |
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Principal Investigator |
TSURUMI Tatsuya 愛知県がんセンター(研究所), 腫瘍ウイルス学部, 部長 (90172072)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 複製 / ウイルス / 転写 / アセンブリー |
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| Research Abstract |
Productive replication of the Epstein-Barr virus (EBV) occurs in discrete sites in nuclei, called replication compartments, where viral genome DNA synthesis, transcription, and encapsidation take place. The replication compartments include subnuclear domains, designated BMRF1 cores, which are highly enriched in the BMRF1 protein. During viral lytic replication, newly synthesized viral DNA genomes are organized around and then stored inside BMRF1 cores. We determined spatial distribution of viral early and late gene mRNAs within replication compartments. EBV early mRNAs were mainly located outside the BMRF1 cores, while viral late mRNAs were identified inside, corresponding well with the fact that late gene transcription is dependent on viral DNA replication. Further, the encapsidation occurs inside in innermost areas of BMRF1-cores.
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[Journal Article] Role of down-regulated neutral ceramidase during all-trans retinoic acid induced neuronal differentiation in SH-SY5Y neuroblastoma cells2012
Author(s)
Tanaka K, Tamiya-Koizumi K, Hagiwara K, Ito H, Takagi A, Kojima T, Suzuki M, Iwaki S, Fujii S, Nakamura M, Banno Y, Kannagi R, Tsurumi T, Kyogashima M, Murate T.
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Journal Title
J Biochem
Volume: 151
Pages: 611-620
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