Analysis of Immune Memory Dynamics by visualizing memory B cell subsets

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659222
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: ISE Wataru 大阪大学, 免疫学フロンティア研究センター, 准教授 (70323483)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: B細胞 / 免疫記憶 / 抗体産生応答

Research Abstract

We have established a new mouse line in which activated IgM+ B cells are specifically labeled and can be inducibly depleted. The M-SDV mice have loxp-flanked STOP cassette followed by DTR-Venus expression construct in Ig heavy chain Cmu locus. If Cre-mediated recombination occurs, IgM+ B cells should express DTR-Venus fusion protein. We crossed M-SDV line with AID-cre. After immunization, a fraction of IgM+ B cells became DTR-Venus positive, while IgM- B cells remained DTR-Venus negative. Further, when the mice were administered with DT, the DTR-Venus positive cells were completely deleted. These results suggest that M-SDV mouse model is an excellent system to detect or deplete IgM memory B cells whose function is largely unknown.