2013 Fiscal Year Final Research Report
Development of target-specific multifunctional exosomes loaded with RNA drugs
| Project/Area Number |
24659260
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied pharmacology
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKAHASHI Yuki 京都大学, 薬学研究科(研究院), 助教 (00547870)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Yoshinobu 京都大学, 大学院薬学研究科, 教授 (30171432)
NISHIKAWA Makiya 京都大学, 大学院薬学研究科, 准教授 (40273437)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ドラッグデリバリー / エキソソーム / ルシフェラーゼ / イメージング / DDS |
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| Research Abstract |
In the present study, in vivo fate of exosomes was analyzed for the goal of the development of exosome-based drug delivery system. B16BL6 murine melanoma cells were selected as mode exosome-producing cells. It was found that intravenously administered exosomes quickly disappeared from blood circulation with short half-life of approximately 2 minutes. Exosomes distributed mainly to the liver, lung and spleen. In addition, it was demonstrated that quick clearance of exosomes from blood circulation was caused by the uptake of exosomes by macrophages in the liver and spleen.
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