2013 Fiscal Year Final Research Report
Establishment of new extraction system from bone marrow smear by laser microdissection
| Project/Area Number |
24659273
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
ADACHI Souichi 京都大学, 医学(系)研究科(研究院), 教授 (10273450)
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| Co-Investigator(Renkei-kenkyūsha) |
ITOU Hiroshi 京都大学, 医学研究科, 助教 (20362387)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 遺伝子解析 / 血液標本 / 白血病 / 骨髄異形成症候群 / 新規予後因子 |
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| Research Abstract |
We established a new laser microdissection system extracting cDNA and mRNA of leukemic blasts from smear of bone marrow samples of transient abnormal myelopoiesis (TAM) or acute myeloid leukemia with Down syndrome (AML-DS). We could detect same GATA1 mutation in the TAM patient both from bone marrow samples and from the microdissecton system. We could also detect GAPDH from mRNA through this microdissection system and measured CXCR4 expression by RT-PCR. We will be able to detect new prognostic factors from AML or MDS patients by next generation sequencing through this microdissection system.
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