2012 Fiscal Year Final Research Report
Use of peptide nucleic acid probes for pathogen-targeted metagenomics
| Project/Area Number |
24659275
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Osaka University |
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Principal Investigator |
YASUNAGA Teruo 大阪大学, 微生物病研究所, 教授 (20260630)
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| Co-Investigator(Kenkyū-buntansha) |
KAIHATSU Kunihiro 大阪大学, 産業科学研究所, 助教 (70419464)
NAKAMURA Shota 大阪大学, 微生物病研究所, 助教 (90432434)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYA Takaaki 京都府立医科大学, 医学研究科, 教授 (80271633)
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| Project Period (FY) |
2012
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| Keywords | 遺伝子検査学 |
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| Research Abstract |
Metagenomics is becoming an important tool for comprehensive pathogen detection from clinical specimens. So far, using this methodology, we have detected pathogenic bacteria, influenza viruses and noroviruses from clinical samples. In this study, to increase the sensitivity and efficiency of our method, we developed a basic protocol for influenza-targeted-metagenomics using a peptide nucleic acid (PNA) probe. A PNA probe that recognizes a conserved sequence among influenza A viruses was designed and synthesized. The efficiency of the probe for target capturing was assessed using a mouse model for influenza infection. We show that treatment of the probe results in a 27-fold increase in the ratio of mouse genome to target DNA, compared to a 7-fold increase without the use of the probe. The protocol established here will serve as a basis for pathogen-targeted metagenomics.
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[Journal Article] Kaihatsu K, Sawada S, Nakamura S, Nakaya T, Yasunaga T, Kato N. Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic2013
Author(s)
Kaihatsu K, Sawada S, Nakamura S, Nakaya T, Yasunaga T, Kato N
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Journal Title
Acid. PLoS One
Volume: 8(5)
Pages: e64017
DOI
Peer Reviewed
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