2014 Fiscal Year Final Research Report
Development of a novel method to detect circulating tumor cells (CTCs) by expression of tissue specific mRNAs in peripheral blood.
| Project/Area Number |
24659277
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
|
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| Research Institution | Takarazuka University |
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Principal Investigator |
|
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | LAMP / CTC |
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| Outline of Final Research Achievements |
To detect circulating tumor cells (CTCs) by expression of tissue specific mRNAs in peripheral blood using Loop-mediated isothermal amplification method (LAMP), expression of thyroid specific genes were studied. Low level expression of mRNAs of one thyroid specific gene was detected by LAMP after reverse transcription (RT-LAMP) in total RNAs from leukocytes. Thus measuring expression levels of tissue specific mRNAs in whole peripheral blood using RT-LAMP may be able to detect CTCs in peripheral blood.
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| Free Research Field |
臨床検査学
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