Development of a reliable semiquantitative screening method for forensic samples

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659338
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Kyushu University
• Principal Investigator: KUDO KDIKO 九州大学, 医学(系)研究科(研究院), 講師 (10186405)
• Co-Investigator(Kenkyū-buntansha): HAYASHIDA Makiko 日本医科大学, 医学部, 准教授 (60164977) ; SAKA Kanju 東京大学, 医学系研究科 (30447388)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 法医中毒学 / 薬毒物スクリーニング / NAGINATA / GC-MS / LC-MS/MS

Research Abstract

We have developed a rapid procedure for simultaneous extraction of a wide range of acidic and basic drugs from whole blood samples for reliable semi-quantitative drug screening by GC-MS. To extract the wide range of drugs, the partition/extraction procedure used for the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) method was selected at the initial step and the protein-lipid removal filter cartridge (Captiva ND Lipids) was found to be useful to remove interference compounds to obtain clean extracts as the second step to be connected to GC-MS screening using the NAGINATA software with our constructed calibration-locking database. We also examined the applicability of our established method in an actual forensic case. The established preparation procedure was applied to tissue samples with some modification, and it was also suitable for LC-MS/MS screening.

Research Products

• [Journal Article] Rapid and simultaneous extraction of acidic and basic drugs from human whole blood for reliable semi-quantitative NAGINATA drug screening by GC-MS (2014)
  • Author(s): K. Kudo, Y. Usumoto, K. Usui, M. Hayashida, E. Kurisaki, K. Saka, A. Tsuji, N. Ikeda
  • Journal Title: Forensic Toxicol Volume: 32 Pages: 97-104
  • Peer Reviewed
• [Journal Article] 薬毒物分析における超高速LC-MS/MSの有用性 (2014)
  • Author(s): 箕畑俊和,工藤恵子,臼井聖尊,志摩典明,片木宗弘,池田典昭,土橋均,鈴木廣一
  • Journal Title: 島津評論 Volume: 70 Pages: 149-155
• [Journal Article] Relationship between the matrix effect and physicochemical properties of analytes in gas chromatography (2013)
  • Author(s): K. Saka, K. Kudo, M. Hayashida, E. Kurisaki, H. Niitsu, M. Terada, K. Yamaguchi, K. Yoshida
  • Journal Title: Anal Bioanal Chem Volume: 405 Pages: 9879-9888
  • Peer Reviewed
• [Presentation] Rapid and simultaneous extraction of acidic and basic drugs using modified QuEChERS method and protein–lipid removal filter cartridge (2014)
  • Author(s): K. Kudo, Y. Usumoto, K. Usui, M. Hayashida, E. Kurisaki, K. Saka, A. Tsuji, N. Ikeda
  • Organizer: 9^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20140000
• [Presentation] Rapid screening and semi-quantitative analysis for forensic drugs in blood using liquid chromatography triple quadrupole mass spectrometry (2013)
  • Author(s): H. Thomas, K. Kudo, T. Minohata, K.. Usui, N. Shima, M. Katagi , H. Tsuchihashi, K. Suzuki, I. Hirano, N Ikeda
  • Organizer: 61th ASMS Conference on Mass Spectrometry; Abstracts
  • Place of Presentation: Minneapolis, Minnesota, USA
  • Year and Date: 20130000
• [Presentation] 薬毒物スクリーニング時、同時定量法の開発―前処理法の再検討 (2013)
  • Author(s): 工藤恵子,臼元洋介,鮫島直美,辻彰子,池田典昭
  • Organizer: 第97次日本法医学会学術全国集会
  • Place of Presentation: 札幌
  • Year and Date: 20130000
• [Presentation] MDMA及びその活性代謝物(DHMA, DHA)のLC-MS/MS同時定量のための前処理法の検討 (2013)
  • Author(s): 坂幹樹,新谷香,山口晃志,林田眞喜子,大野曜吉,吉田謙一
  • Organizer: 第82回日本法医学会学術関東地方会
  • Place of Presentation: 横浜
  • Year and Date: 20130000
• [Presentation] ベンゾジアゼピン系薬物を含む剖検薬物事例に対する包括的QTOFスクリーニングの応用 (2013)
  • Author(s): 林田眞喜子,滝埜昌彦,山口晃志,植草協子,安部寛子,大野曜吉
  • Organizer: 第97次日本法医学会学術全国集会
  • Place of Presentation: 札幌
  • Year and Date: 20130000
• [Presentation] Rapid diagnosis of drug intoxication by standard free GC-MS method : construction and method evaluation using forensic samples (2012)
  • Author(s): K. Kudo, Y. Usumoto, N. Sameshima. A. Tsuji, N. Ikeda
  • Organizer: The 50^<th> annual meeting of the International Association of Forensic Toxicologists. Abstracts
  • Place of Presentation: Hamamatsu
  • Year and Date: 20120000
• [Presentation] Development of a generic approach to drugs of abuse screening using fast polarity switching MRM triggered product ion scanning on the fly (2012)
  • Author(s): T. Minohata, J. Iida, K. Kudo, N. Ikeda, K. Zaoysu, N. Shima, M. Katagi, H. Tsuchihashi, K. Suzuki
  • Organizer: The 50^<th> annual meeting of the International Association of Forensic Toxicologists. Abstracts
  • Place of Presentation: Hamamatsu
  • Year and Date: 20120000
• [Presentation] Screening analysis for drugs of abuse by LC-MS/MS enables fast polarity switching MRM triggered product ion scanning on the fly (2012)
  • Author(s): K Kudo, T Minohata, J Iida, H Tsuchihashi, K Suzuki, K Zaitsu, N Shima, M Katagi, N Ikeda
  • Organizer: 60th ASMS Conference on Mass Spectrometry; Abstracts
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20120000
• [Presentation] Screening analysis for drugs of abuse by LC-MS/MS enables fast polarity switching MRM triggered product ion scanning on the fly. 2012 p116 (2012)
  • Author(s): T Minohata, I Hirano, J Iida, K Kudo, N Ikeda, K Zaitsu, N Shima, M Katagi, H Tsuchihashi, K Suzuki
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: Kyoto,Japan
  • Year and Date: 20120000
• [Presentation] 解剖事例のなかでの薬毒物スクリーニングの役割 (2012)
  • Author(s): 工藤恵子
  • Organizer: 第96回法中毒懇話会
  • Place of Presentation: 浜松
  • Year and Date: 20120000
• [Presentation] 法医学試料のための標準的薬毒物スクリーニングシステムの構築 (2012)
  • Author(s): 工藤恵子,池田典昭
  • Organizer: 第37回日本医用マススペクトル学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 20120000
• [Remarks] ガスクロマトグラフィー・質量分析用薬毒物データベース 著作権:九州大学所有(QC80006)
• [Remarks] 工 藤恵子.あなたも使ってみたくなる!固相抽出法の基礎の基礎.第2回法医中毒研究会セミナー「薬物抽出法の基本を学ぶ」セミナーテキスト. 2013: p30-36,京都
• [Remarks] 工 藤恵子. NAGINATA-GC/MSスクリーニングのポイントと今後の課題.第2回NAGINATA-GC/MSユーザーセミナーテキスト. 2014: p1-13,東京