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2013 Fiscal Year Final Research Report

Analysis of the cell neoplastic transformation mechanism based on the molecular chaperone HSP90

Research Project

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Project/Area Number 24659357
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionAkita University

Principal Investigator

ITOH Hideaki  秋田大学, 工学(系)研究科(研究院), 教授 (80168369)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords分子シャペロン / DNAポリメラーゼη
Research Abstract

Deficiency of DNA Pol eta in humans causes a variant form of the cancer predisposition syndrome xeroderma pigmentosum.
We will clarify the physiological significance of HSP90 and Pol eta interaction. We tried the construction and purification of full-length human Pol eta. Pol eta is a very unstable protein, but by the addition of a His-tag in the C-terminal, we have succeeded in purification of Pol eta. Also, we got specific polyclonal antibody against Pol eta. HSP effected on the stability of Pol eta. HSP90 and Pol eta were co-localized strongly near nuclear of glyoblastoma cells. Glyoblastoma is the most aggressive malignant primary brain tumor in humans. On the contrary, we couldn't clear interaction between HSP90 and Pol eta in the ependymoma cells. Ependymoma is a tumor that arises from the ependyma, a tissue of the central nervous system.

  • Research Products

    (2 results)

All 2013

All Presentation (2 results)

  • [Presentation] HSP90とDNA polymeraseηの相互作用解析2013

    • Author(s)
      羽賀愛沙美,岡本知也,畠山詩織,菅原卓,南條博,伊藤英晃
    • Place of Presentation
      神戸市
    • Year and Date
      20131203-06
  • [Presentation] DNA polymeraseηとHSP90の相互作用解析2013

    • Author(s)
      羽賀愛沙美,岡本知也,畠山詩織,黒澤友翼,三瓶杏,西聡美,菅原卓,南條博,伊藤英晃
    • Organizer
      第8回臨床ストレス応答学会大会
    • Place of Presentation
      信州大学
    • Year and Date
      2013-11-15

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Published: 2015-06-25  

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