2012 Fiscal Year Final Research Report
New approach to analyze inherited arrhythmias using human iPS cells
| Project/Area Number |
24659387
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
MAKIYAMA Takeru 京都大学, 医学研究科, 助教 (30528302)
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| Project Period (FY) |
2012
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| Keywords | 不整脈 / 分子心臓病態学 |
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| Research Abstract |
In this study, we investigated the disease causing mechanisms of inherited arrhythmias using human iPS cell derived cardiomyocytes; 1. We assessed the ultrastructural changes of hiPS-CMs during a one-year culture and demonstrated the ultrastructural sarcomeric maturation process. 2. We generated disease-specific iPS cells from a patient associated with catecholaminergic polymorphic ventricular tachycardia. The differentiated cardiomyocytes had an increased susceptibility to catecholamine-induced calcium waves indicating that the cells recapiturated the CPVT phenotype. 3. We generated iPS cells and conducted whole exome sequencing in a large family with an inherited arrhythmia (under analysis).
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Research Products
(14 results)
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[Journal Article] Ultrastructural Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a Long-Term Culture2013
Author(s)
Kamakura T, Makiyama T, Sasaki K, Yoshida Y, Wuriyanghai Y, Chen J, Hattori T, Ohno S, Kita T, Horie M, Yamanaka S, Kimura T
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Journal Title
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[Journal Article] Long-Term Follow-Up of a Pediatric Cohort With Short QT Syndrome2013
Author(s)
Villafane J, Atallah J, Gollob MH, Maury P, Wolpert C, Gebauer R, Watanabe H, Horie M, Anttonen O, Kannankeril P, Faulknier B, Bleiz J, Makiyama T, Shimizu W, Hamilton R, Young ML
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Journal Title
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[Journal Article] Novel SCN3B Mutation Associated With Brugada Syndrome Affects Intracellular Trafficking and Function of Nav1.52012
Author(s)
Ishikawa T, Takahashi N, Ohno S, Sakurada H, Nakamura K, On YK, Park JE, Makiyama T, Horie M, Arimura T, Makita N, Kimura A
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Journal Title
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[Journal Article] A novel gain-of-function KCNJ2 mutation associated with short QT syndrome impairs inward rectification of Kir2.1 currents.2012
Author(s)
Hattori T, Makiyama T, Akao M, Ehara E, Ohno S, Iguchi M, Nishio Y, Sasaki K, Itoh H, Yokode M, Kita T, Horie M, Kimura T
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Journal Title
Cardiovasc Res
Volume: 93(4)
Pages: 666-73
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[Journal Article] Phenotype variability in patients carrying KCNJ2 mutations.2012
Author(s)
Kimura H, Zhou J, Kawamura M, Itoh H, Mizusawa Y, Ding WG, Wu J, Ohno S, Makiyama T, Miyamoto A, Naiki N, Wang Q, Xie Y, Suzuki T, Tateno S, Nakamura Y, Zang WJ, Ito M, Matsuura H, Horie M
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Journal Title
Circ Cardiovasc Genet.
Volume: 5(3)
Pages: 344-53
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[Journal Article] Clinical characteristics and risk of arrhythmia recurrences in patients with idiopathic ventricular fibrillation associated with early repolarization.2012
Author(s)
Watanabe H, Nogami A, Ohkubo K, Kawata H, Hayashi Y, Ishikawa T, Makiyama T, Nagao S, Yagihara N, Takehara N, Kawamura Y, Sato A, Okamura K, Hosaka Y, Sato M, Fukae S, Chinushi M, Oda H, Okabe M, Kimura A, Maemura K, Watanabe
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Journal Title
Int J Cardiol
Volume: 159(3)
Pages: 238-40
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