2012 Fiscal Year Final Research Report
The role of sarcomere-specific isoform of docking protein Gab1in heart failure
| Project/Area Number |
24659391
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KOMURO Issei 東京大学, 医学系研究科, 教授 (30260483)
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| Project Period (FY) |
2012
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| Keywords | Gab1 / ドッキングタンパク質 / neuregulin-1 / サルコメア / アイソフォーム |
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| Research Abstract |
We are focusing on the role of Gab docking proteins in the cardiovascular field. In the present study, we identified a novel isoformof Gab1 (120 kDa) expressed exclusively in cardiac muscle and skeletal muscle, which is different from a ubiquitous isoform of Gab1 expressed in all the tissues. We succeeded in cloning of sarcomere-specific Gab1 isoform cDNA and found the existence of extra-exon in sarcomere-specific Gab1 isoform in mammals. RT-PCR analysis revealed that the expression of this extra-exon was confined to myocardium and skeletal muscle in mice. In addition, we created rabbit polyclonal antibody specific to the amino acid sequences of this extra-exon, and found that this isoform is indeed expressed in the embryonic murine heart at E12.5.
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