2013 Fiscal Year Final Research Report
Role of aquaporins in endoplasmic reticulum
| Project/Area Number |
24659413
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SASAKI SEI 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60170677)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 水・電解質代謝学 / 水チャネル / 遺伝子改変マウス |
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| Research Abstract |
The purpose of our study was to clarify pathopysiologival roles of aquaporins in endoplasmic reticulum (ER). AQP11 knockout mice (AQP11-KO) showed polycystic kidney. However, molecular mechanism(s) of the cyst formation remained to be determined. To clarify this, we generated HA-tagged AQP11 BAC transgenic mice (AQP11-TG), and also analyzed AQP11-KO focusing on PKD1 (PC-1)and PKD2 (PC-2). AQP11 was found to be localized in endoplasmic reticulum in the proximal tubules, and impaired glycosylation processing and aberrant membrane trafficking of PC-1 was observed in AQP11 KO mice, suggesting the involvement of AQP11 in proper glycosylation of PC-1 in ER.
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Research Products
(29 results)
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[Journal Article] Development of enzyme-linked immunosorbent assays for urinary thiazide-sensitive Na-Cl cotransporter (NCC) measurement2013
Author(s)
Isobe K, Mori T, Asano T, Kawaguchi H, Nonoyama S, Kumagai N, Kamada F, Morimoto T, Hayashi M, Sohara E, Rai T, Sasaki S, Uchida S.
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Journal Title
Am J Physiol Renal Physiol
Volume: 305
Pages: F1374-81
Peer Reviewed
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[Journal Article] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension2013
Author(s)
Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Matsuo H, Murata T, Nomura S, Asano T, Kawaguchi H, Nonoyama S, Rai T, Sasaki S, Uchida S.
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Journal Title
Cell Rep
Volume: 3
Pages: 858-68
Peer Reviewed
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[Journal Article] Dietary salt intake regulates WNK3-SPAK-NKCC1 phosphorylation cascade in mouse aorta through angiotensin II2013
Author(s)
Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S.
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Journal Title
Hypertension
Volume: 62
Pages: 872-878
Peer Reviewed
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[Journal Article] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination2012
Author(s)
Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Oi K, Rai T, Sasaki S, Uchida S.
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 425
Pages: 456-61
Peer Reviewed
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[Journal Article] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels2012
Author(s)
Susa K, Kita S, Iwamoto T, Yang SS, Lin SH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S.
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Journal Title
Clin Exp. Nephrol
Volume: 16
Pages: 530-8
Peer Reviewed
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[Presentation] Impaired KLHL3-mediated ubiquitination of WNK4 activates OSR1 and SPAK kinases-NaCl cotransporter (NCC) signaling and causes hypertension2013
Author(s)
Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Rai T, Sasaki S, Uchida S.
Organizer
The 46^<th> Annual Meeting of American Society of Nephrology
Place of Presentation
Atlanta
Year and Date
2013-11-09
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[Presentation] WNK3 Regulates Blood Pressure Through the Regulation of Vascular OSR1/SPAK-NKCC1 Phosphorylation Cascade2013
Author(s)
Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S.
Organizer
The 46^<th> Annual Meeting of American Society of Nephrology
Place of Presentation
Atlanta
Year and Date
2013-11-09
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