Role of fatty acid elongase Elovl6 in the sucrose preference formation

2012 Fiscal Year Final Research Report

• Project/Area Number: 24659441
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: University of Tsukuba
• Principal Investigator: SHIMANO Hitoshi 筑波大学, 医学医療系, 教授 (20251241)
• Co-Investigator(Kenkyū-buntansha): MATSUZAKA Takashi 筑波大学, 医学医療系, 准教授 (70400679) ; NAKAGAWA Yoshimi 筑波大学, 医学医療系, 講師 (80361351)
• Project Period (FY): 2011 – 2012
• Keywords: 脂肪酸 / 嗜好性

Research Abstract

We investigated the effects of Elovl6 deficiency on the food intake and food preference in mice. Three types of diets were used in this study, with one or two diets simultaneously available to wild-type (WT) and Elovl6 knockout (KO) mice. In Experiment 1, mice were fed a standard chow, high fat, or high sucrose diet. In Experiment 2, WT and Elovl6KO mice were fed one of three pairs of diet, chow and HF, HS and HF, or chow and HS. Food intake was sequentially monitored in individually caged mice. In Experiment 1, Elovl6KO mice showed an enhanced ingestion of HS diet than WT mice. In contrast, the standard chow and HF diet intake were similar between WT and Elovl6KO mice. In Experiment 2, as compared with WT mice, Elovl6KO mice showed a greater preference for HS diet than a chow diet, but exhibited an equivalent preference for chow versus HF diet, or HS versus HF diet. With chow and HS diets, the total amount of food and calorie intake, which are the sum of HS and chow diet, were similar between WT and Elovl6 KO mice because chow diet consumption was decreased at all time point in Elovl6KO mice. Elovl6KO mice demonstrated a clear enhanced preference for HS diet. These results indicate that Elovl6 may play a specific and unique role in the control of hedonic eating such as sucrose palatability, but have no effect on homeostatic eating.

Research Products

• [Journal Article] TFE3 inhibits myoblast differentiation inC2C12 cells via down-regulating geneexpression of myogenin (2013)
  • Author(s): Naka A, Iida KT, Nakagawa Y, IwasakiH, Takeuchi Y, Satoh A, Matsuzaka T, IshiiKA, Kobayashi K, Yatoh S, Shimada M, YahagiN, Suzuki H, Sone H, Yamada N, Shimano H
  • Journal Title: Biochem BiophysRes Commun Volume: 430(2) Pages: 664-669
  • Peer Reviewed
• [Journal Article] Elovl6 promotes nonalcoholic steatohepatitis (2012)
  • Author(s): Matsuzaka T, Atsumi A, Matsumori R, Nie T, Shinozaki H, Suzuki-Kemuriyama N, Kuba M, Nakagawa Y, Ishii K, Shimada M, Kobayashi K, Yatoh S, Takahashi A,Takekoshi K, Sone H, Yahagi N, Suzuki H, Murata S, Nakamuta M, Yamada N, Shimano H
  • Journal Title: Hepatology Volume: 56(6) Pages: 2199-2208
  • Peer Reviewed
• [Journal Article] Dicer hasa crucial role in the early stage ofadipocyte differentiation, but not inlipid synthesis, in 3T3-L1 cells (2012)
  • Author(s): Fujimoto Y, Nakagawa Y, Shingyouchi A,Tokushige N, Nakanishi N, Satoh A,Matsuzaka T, Ishii KA, Iwasaki H,Kobayashi K, Yatoh S, Suzuki H, Yahagi N,Urayama O, Yamada N, Shimano H
  • Journal Title: BiochemBiophys Res Commun Volume: 420(4) Pages: 931-936
  • Peer Reviewed
• [Journal Article] regulates muscle metabolic geneexpression, increases glycogen stores,and enhances insulin sensitivity in mice. (2012)
  • Author(s): Iwasaki H, Naka A, Iida KT, NakagawaY, Matsuzaka T, Ishii KA, Kobayashi K,Takahashi A, Yatoh S, Yahagi N, Sone H,Suzuki H, Yamada N, Shimano H
  • Volume: 302(7)
  • Pages: E896-902
  • Peer Reviewed
• [Presentation] アディポサイエンスからみた動脈硬化性疾患予防の展望 (2013)
  • Author(s): 島野 仁
  • Organizer: 第13回動脈硬化教育フォーラム
  • Place of Presentation: 国立京都国際会館、京都
  • Year and Date: 2013-02-03
• [Presentation] Deletion of Elovl6 protects against thefailure of pancreatic beta-cell mass andfunction in db/db mice (2012)
  • Author(s): Yuta Nakano, Takashi Matsuzaka, NieTang, Marii Suzuki, Noriko Suzuki-Kemuriyama, Motoko Kuba, KeitaShoshi, Tomotaka Yokoo, Yoshimi Nakagawa,Kiyoaki Ishii, Masako Shimada, HitoshiIwasaki, Kazuto Kobayashi, Shigeru Yatoh,Akimitsu Takahashi, Naoya Yahagi, Hi
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡、福岡
  • Year and Date: 2012-12-11
• [Presentation] Elovl6 promotesnonalcoholic steatohepatitis in mice andhumans (2012)
  • Author(s): Takashi Matsuzaka, Tang Nie, NorikoSuzuki-Kemuriyama, Motoko Kuba, YoshimiNakagawa, Kiyoaki Ishii, Masako Shimada,Kazuto Kobayashi, Shigeru Yatoh, AkimitsuTakahashi, Hirohito Sone, Naoya Yahagi,Hiroaki Suzuki, Makoto Nakamuta, NobuhiroYamada, Hitoshi Shim
  • Organizer: 9th IDF-WPR Congress and 4th AASDScientific Meeting
  • Place of Presentation: 国立京都国際会館, 京都
  • Year and Date: 2012-11-26
• [Presentation] 糖尿病へのアプローチ:病態メカニズムオリエンテッド とエネルギー代謝ネットワーク的視点 (2012)
  • Author(s): 島野 仁
  • Organizer: 第1回日本くすりと糖尿病学会学術集会
  • Place of Presentation: 星薬科大学 本館メインホール・新星館
  • Year and Date: 2012-09-23
• [Presentation] マクロファージ泡沫化および動脈硬化における脂肪酸伸長酵素Elovl6の役割 (2012)
  • Author(s): 松坂 賢、齋藤 亮、唐澤 直義、松森 理恵、煙山 紀子、中川 嘉、石井 清朗、岩崎 仁、小林 和人、矢藤 繁、高橋 昭光、曽根 博仁、鈴木 浩明、矢作 直也、山田 信博、島野 仁
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2012-05-19
• [Presentation] 脂質の質に視点をおいた脂質代謝疾患治療戦略 (2012)
  • Author(s): 島野 仁
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2012-04-21
• [Remarks]
  • URL: http://www.u-tsukuba-endocrinology.jp/