2012 Fiscal Year Final Research Report
Pathophysiological elucidation of refractory leukemias with the genetic analyses at the single-cell level
| Project/Area Number |
24659457
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KUROKAWA Mineo 東京大学, 医学部附属病院, 教授 (80312320)
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| Project Period (FY) |
2012
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| Keywords | 白血病幹細胞 / BCR-ABL / clonal evolution |
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| Research Abstract |
In this study, we assessed the variations of BCR-ABL expression at the single-cell level, with the use of patient samples from a t(3;21) positive chronic myeloid leukemia patient and a single-cell gene expression analyzer. We assessed several cell populations of patient samples, isolated via surface antigen profiles. In the result, we found that the expression level of BCR-ABL in CD34+/ CD38-/CD90+ leukemic cells was likely to be lower than those in other fractions at the single-cell level.
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